Journal of Structural BiologyNeurosciencePeer-reviewed journal article
Electron cryo-tomography of vestibular hair-cell stereocilia
Zoltan Metlagel, Jocelyn F. KreyiD, Junha SongiD, Mark F. SwiftiD, William J. Tivol, Rachel A. DumontiD, Jasmine Thai, Alex Chang, Helia SeifikariD, Niels VolkmanniD, Dorit HaneiniD, Peter G. GillespieiD, Manfred AueriD
Abstract
High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which allowed subsequent 3D imaging by electron cryo-tomography. Structures of stereocilia tips, shafts, and tapers were revealed, demonstrating that the actin paracrystal was not perfectly ordered. This sample-preparation and imaging procedure will allow for examination of structural features of stereocilia in a near-native state.
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