Dual-view oblique plane microscopy (dOPM)

We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a water immersion 40x 1.15 NA primary objective, deconvolved image volumes of 200 nm beads were measured to have full width at half maxima (FWHM) of 0.35{+/-}0.04 m and 0.39{+/-}0.02 m laterally and 0.81{+/-}0.07 m axially. The laterally integrated z-sectioning value was 1.33{+/-}0.45 m using light-sheet FWHM in the frames of the two views of 4.99{+/-}0.58 m and 4.89{+/-}0.63 m. To qualitatively demonstrate that the system can reduce shadow artefacts while providing a more isotropic resolution, a multi-cellular spheroid approximately 100 m in diameter was imaged.