R. P. Fiolka

Adaptive Optics in an Oblique Plane Microscope

This paper demonstrates adaptive optics in an oblique plane microscope, improving resolution and signal strength in zebrafish embryos.

Imaging of cellular dynamics in vitro and in situ: from a whole organism to sub-cellular imaging with self-driving, multi-scale microscopy

The paper introduces a self-driving, multi-resolution light-sheet microscope to simultaneously observe sub-cellular dynamics and whole organisms over extended periods, applying it to study development, cancer, and immune interactions in zebrafish.

navigate: an open-source platform for smart light-sheet microscopy.

navigate provides an open-source platform for smart light-sheet microscopy, enabling automated and user-friendly LSFM workflows without requiring specialized expertise.

Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring

The paper introduces a mesoscopic oblique plane microscope for whole-organism imaging with high speed and subcellular resolution, demonstrating its application in zebrafish vasculature and blood flow analysis.

Projective oblique plane structured illumination microscopy

This paper introduces OPSIM, a structured illumination microscopy technique that enables rapid whole-cell imaging with doubled resolution, achieving up to 2.7 Hz for mitochondrial and ER dynamics.

Resolution doubling in light-sheet microscopy via oblique plane structured illumination

This paper demonstrates isotropic resolution doubling in light-sheet microscopy using oblique plane structured illumination, achieving sub-150nm lateral resolution with reduced phototoxicity and faster volumetric imaging.

Increasing the Field-of-View in Oblique Plane Microscopy via optical tiling

This paper introduces a dual-axis scan unit for oblique plane microscopy to increase field-of-view and imaging speed, enabling high-resolution volumetric imaging of zebrafish embryos.

Compression-dependent microtubule reinforcement comprises a mechanostat which enables cells to navigate confined environments

The paper shows that microtubules form a mechanostat to coordinate contractility and nuclear positioning during confined cell migration.

Blebs Promote Cell Survival by Assembling Oncogenic Signaling Hubs

This study shows blebs form signaling hubs that promote anoikis resistance in cancer cells by recruiting septins and activating ERK/PI3K pathways.

Heterozygous mutation of Vegfr3 decreases renal lymphatics but is dispensable for renal function

This study shows that heterozygous Vegfr3 mutation reduces renal lymphatics but does not impair renal function or histology, even after cisplatin injury.

u-track 3D: measuring and interrogating dense particle dynamics in three dimensions.

u-track 3D provides a software package for analyzing dense 3D particle dynamics in microscopy data by improving visualization, trajectory validation, and interactive exploration.

Proteolysis-free cell migration through crowded environments via mechanical worrying

The paper shows amoeboid melanoma cells migrate through crowded environments by mechanically degrading extracellular matrix via bleb-driven abrading, without proteolysis.

Real-Time Multi-Angle Projection Imaging of Biological Dynamics

This paper introduces a projection imaging method that enables real-time, multi-angle visualization of biological processes with reduced data overhead.

A Versatile Oblique Plane Microscope for Large-Scale and High-Resolution Imaging of Subcellular Dynamics.

This paper introduces an improved oblique plane microscope with enhanced resolution and usability, demonstrating its application in imaging various subcellular dynamics and challenging biological phenomena.

Converting lateral scanning into axial focusing to speed up 3D microscopy

This paper introduces an optical design that converts lateral scanning into axial focusing, enabling rapid, high-resolution 3D imaging with axially swept light-sheet microscopy.

The nucleus acts as a ruler tailoring cell responses to spatial constraints

The paper shows that the nucleus acts as a ruler by triggering contractile responses when confined, influencing cell migration and processes like cancer invasion and immune responses.

Concepts for structured illumination microscopy with extended axial resolution through mirrored illumination

This paper proposes a simplified dual-objective structured illumination microscopy method to improve axial resolution without interferometric detection.

Light-sheet engineering using the Field Synthesis theorem

This paper introduces C-light-sheets, a method to continuously tune light-sheet thickness and illumination confinement using the Field Synthesis theorem for improved imaging.

Universal Light-Sheet Generation with Field Synthesis

This paper introduces Field Synthesis, a theorem that simplifies light-sheet generation for microscopy, enabling high-throughput, multicolor imaging with reduced photobleaching.