B.-J. Chang

Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring

The paper introduces a mesoscopic oblique plane microscope for whole-organism imaging with high speed and subcellular resolution, demonstrating its application in zebrafish vasculature and blood flow analysis.

Projective oblique plane structured illumination microscopy

This paper introduces OPSIM, a structured illumination microscopy technique that enables rapid whole-cell imaging with doubled resolution, achieving up to 2.7 Hz for mitochondrial and ER dynamics.

Resolution doubling in light-sheet microscopy via oblique plane structured illumination

This paper demonstrates isotropic resolution doubling in light-sheet microscopy using oblique plane structured illumination, achieving sub-150nm lateral resolution with reduced phototoxicity and faster volumetric imaging.

Increasing the Field-of-View in Oblique Plane Microscopy via optical tiling

This paper introduces a dual-axis scan unit for oblique plane microscopy to increase field-of-view and imaging speed, enabling high-resolution volumetric imaging of zebrafish embryos.

In vivo profiling of site-specific human cancer cell states in zebrafish

The paper uses zebrafish xenografts and computer vision to study how Ewing Sarcoma cell morphology changes in different sites and under EWSR1-FLI1 expression variations.

Blebs Promote Cell Survival by Assembling Oncogenic Signaling Hubs

This study shows blebs form signaling hubs that promote anoikis resistance in cancer cells by recruiting septins and activating ERK/PI3K pathways.

Real-Time Multi-Angle Projection Imaging of Biological Dynamics

This paper introduces a projection imaging method that enables real-time, multi-angle visualization of biological processes with reduced data overhead.

A systematic and quantitative comparison of lattice and Gaussian light-sheets

This study compares lattice and Gaussian light-sheets quantitatively, finding they are similar in thickness, confocal parameter, and propagation length.

A Versatile Oblique Plane Microscope for Large-Scale and High-Resolution Imaging of Subcellular Dynamics.

This paper introduces an improved oblique plane microscope with enhanced resolution and usability, demonstrating its application in imaging various subcellular dynamics and challenging biological phenomena.

Converting lateral scanning into axial focusing to speed up 3D microscopy

This paper introduces an optical design that converts lateral scanning into axial focusing, enabling rapid, high-resolution 3D imaging with axially swept light-sheet microscopy.

Direct Arp2/3-vinculin binding is essential for cell spreading, but only on compliant substrates and in 3D

The paper investigates the role of actin-binding proteins in cell spreading on 2D and 3D substrates, finding that Arp2/3-vinculin binding is critical for 3D spreading but not 2D.

Light-sheet engineering using the Field Synthesis theorem

This paper introduces C-light-sheets, a method to continuously tune light-sheet thickness and illumination confinement using the Field Synthesis theorem for improved imaging.

Light-sheet microscopy with isotropic, sub-micron resolution and solvent-independent large-scale imaging

This paper introduces ctASLM, a light-sheet microscopy technique with sub-micron isotropic resolution and large-scale imaging, enabling automated cell and subcellular feature detection in cleared tissues.

An image-based assay to quantify changes in proliferation and viability upon drug treatment in 3D microenvironments

This paper introduces an image-based assay to measure drug effects on cell proliferation and viability in 3D microenvironments, showing 3D single cells are as effective as spheroids for drug screening.

Universal Light-Sheet Generation with Field Synthesis

This paper introduces Field Synthesis, a theorem that simplifies light-sheet generation for microscopy, enabling high-throughput, multicolor imaging with reduced photobleaching.