CRISPR Del/Rei: A simple, flexible and efficient pipeline for scarless genome editing
Marah H. Wahbeh, Kyra L. FeueriD, Christian Yovo, Eman RabieiD, Anh-Thu N. Lam, Sara AbdollahiiD, Lindsay J. YoungiD, Bailey RikeiD, Akul UmamageswaraniD, Dimitrios AvramopoulosiD
Abstract
Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.
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