bioRxivMolecular BiologyPreprint
Proteome integral solubility alteration assay for monitoring antibody-antigen cell interactions
Weiqi Lu, Susanna L. LundströmiD, Yaolin Hou, Sourav Mukherjee, Jesper Z. HaeggströmiD, Massimiliano GaetaniiD, Roman A. ZubareviD
Abstract
The Proteome Integral Solubility Alteration (PISA) assay is commonly used to identify and characterize interactions between small molecules and the cellular proteome. In this study, a modified PISA approach identified tumor necrosis factor alpha (TNF) as the antigen of the monoclonal antibody infliximab in activated monocytes - uniquely among [~]8,000 cellular proteins. These results highlight the potential of PISA-based analysis not only for monitoring antibody-antigen interactions in therapeutic development, but also for identifying new antigens and characterizing the initial effector functions of antibodies within complex proteome environments. TeaserModified PISA pinpointed TNF- as Infliximabs antigen among 8000 proteins, highlighting broad potential for therapeutic antigen studies.
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