Author

M. Gaetani

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 5 Cancer Biology· 2 Cell Biology· 2 Microbiology· 1

Signature Assays, Organisms & Methods

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Assay:proteomics· 9 Approach:experimental assay· 8 Research Focus:cell type annotation· 6 Research Focus:drug response / drug discovery· 5 Organism:human· 5 Approach:statistical model· 3 Research Focus:cell signaling & trafficking· 2 Research Focus:differential expression· 2

Frequent Co-Authors

A. A. Saei· 6 shared A. Vegvari· 5 shared X. Zhang· 5 shared H. Gharibi· 4 shared R. A. Zubarev· 4 shared C. Beusch· 3 shared H. Lyu· 3 shared P. Sabatier· 3 shared

Activity by Year

2018-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 37 mutual co-author links

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6 shared papersBiochemistry

A. A. Saei

Mutual co-authors in cluster

Author profile →

10 papers

Recent·Most cited

Gel-Assisted Proteome Position Integral Shift (GAPPIS) Assay Returns Molecular Weight to Shotgun Proteomics and Identifies Novel Caspase 3 Substrates

Z. Meng, A. A. Saei, X. Zhang, H. Lyu, H. Gharibi, S. L. Lundstrom, A. Vegvari, M. Gaetani, R. A. Zubarev

The paper introduces GAPPIS, a method to recover molecular weight information from shotgun proteomics, identifying novel caspase 3 substrates in apoptotic cells.

bioRxiv · Feb 14, 2024 · PreprintBiochemistry

Do light eaters live shorter lives? The case of ultralight Caenorhabditis elegans

X. Zhang, H. Gharibi, C. M. Beusch, Z. Meng, A. Saei Dibavar, M. Gaetani, R. Zubarev

This study shows that ultralight Caenorhabditis elegans, grown on isotopically depleted bacteria, have shorter lifespans despite faster growth and maturation.

bioRxiv · Feb 13, 2024 · PreprintBiochemistry

Merging Multi-OMICs with Proteome Integral Solubility Alteration Unveils Antibiotic Mode of Action

R. Maity, X. Zhang, F. R. Liberati, C. Scribani, F. Cutruzzola, S. Rinaldo, M. Gaetani, J. A. Ainsa, J. Sancho

This study introduces a multi-omics method to distinguish target-specific and off-target effects of antibiotics using proteome solubility in Helicobacter pylori.

bioRxiv · Sep 7, 2023Microbiology

Multifaceted proteome analysis at solubility, redox, and expression dimensions for target identification

A. A. Saei, A. Lundin, H. Lyu, H. Gharibi, H. Luo, J. Teppo, X. Zhang, M. Gaetani, A. Vegvari, R. Holmdahl, S. P. Gygi, R. A. Zubarev

The paper introduces PISA-REX, a high-throughput proteomics method combining redox, expression, and solubility analysis, and demonstrates its application in studying interferon responses in human monocytes and mice.

bioRxiv · Aug 31, 2023Biochemistry

Chemogenetic inhibition of IST1-CHMP1B interaction impairs endosomal recycling and promotes unconventional LC3 lipidation at stalled endosomes

A. Knyazeva, S. Li, D. Corkery, K. Shankar, L. Herzog, X. Zhang, B. Singh, G. Niggemeyer, D. Grill, J. Gilthorpe, M. Gaetani, L.-A. Carlson, H. Waldmann, Y. Wu

This study inhibits the IST1-CHMP1B interaction in ESCRT-III, impairing endosomal recycling and inducing unconventional LC3 lipidation at stalled endosomes.

bioRxiv · Aug 28, 2023Cell Biology

Lineage-specific oncogenes drive growth of major forms of human cancer using common downstream mechanisms

O. Kauko, M. Turunen, P. Pihlajamaa, A. Hakkinen, R. M. Queiroz, M. Paakkonen, S. Ventela, M. Gaetani, S. Lundstrom, A. Murgia, B. Sahu, J. Routila, H. Irjala, J. L. Griffin, K. S. Lilley, , J. Taipale

The paper identifies common downstream mechanisms driven by lineage-specific oncogenes that regulate cell proliferation in various cancers.

bioRxiv · Sep 27, 2022 · PreprintCancer Biology

Mapping the GALNT1 substrate landscape with versatile proteomics tools

A. A. Saei, S. L. Lundstrom, H. Lyu, H. Gharibi, W. Lu, P. Fang, X. Zhang, Z. Meng, J. Wang, M. Gaetani, A. Vegvari, S. P. Gygi, R. A. Zubarev

This study identifies hundreds of novel GALNT1 substrates using proteomics tools, revealing preferences for Thr residues and Pro at positions +3 and +4, and implicating GALNT1 in diverse biological pathways.

bioRxiv · Aug 24, 2022 · PreprintBiochemistry

System-wide Identification and prioritization of Enzyme Substrates by Thermal Analysis (SIESTA)

A. A. Saei, C. Beusch, P. Sabatier, J. Astorga Wells, A. Chernobrovkin, S. Rodin, K. Nareoja, A.-G. Thorsell, T. Karlberg, Q. Cheng, S. L. Lundstrom, M. Gaetani, A. Vegvari, E. S. J. Arner, H. Schuler, R. Zubarev

SIESTA identifies and prioritizes enzyme substrates by detecting thermal stability changes caused by post-translational modifications, demonstrating effectiveness across multiple enzyme systems.

bioRxiv · Jan 21, 2020Biochemistry

Proteome Integral Stability Alteration assay dramatically increases throughput and sensitivity in profiling factor-induced proteome changes

M. Gaetani, P. Sabatier, A. A. Saei, C. Beusch, Z. Yang, S. Lundstrom, R. A. Zubarev

The paper introduces the PISA assay, which improves throughput and sensitivity in profiling factor-induced proteome stability changes by avoiding curve fitting and enabling parameter variation analysis.

bioRxiv · Dec 13, 2018Cancer Biology

ProTargetMiner: A proteome signature library of anticancer molecules for functional discovery

A. A. Saei, A. Chernobrovkin, P. Sabatier, B. Zhang, C. Beusch, U. Guler Tokat, M. Gaetani, A. Vegvari, R. Zubarev

ProTargetMiner provides a proteome signature library of anticancer molecules in A549 cells, revealing drug targets, co-regulation networks, and essential proteins for cellular functions.

bioRxiv · Sep 18, 2018Cell Biology