GABAA receptor-mediated currents and hormone mRNAs in cells expressing more than one hormone transcript in intact human pancreatic islets

Abstract

In pancreatic islets the major cell-types are , {beta} and {delta} cells, secreting the hormones glucagon (GCG), insulin (INS) and somatostatin (SST), respectively. The GABA ({gamma}-aminobutyric acid) signalling system is expressed in human pancreatic islets. We have previously used single-cell RT-PCR in combination with current recordings to correlate expression of single hormone transcript with functional GABAA receptor (iGABAAR) properties in islets. Here we extended these studies to islet cells from non-diabetic and type 2 diabetic donors that express mRNAs for more than one hormone. We detected cells expressing double (/{beta}, /{delta}, {beta}/{delta} cell-types) and triple (/{beta}/{delta} cell-type) hormone transcripts. The most common mixed-identity cell-type was the /{beta} group where the cells could be grouped into {beta}- and -like subgroups. The {beta}-like cells had low GCG/INS expression ratio ( 1.2). The difference in expression levels and single channel iGABAAR characteristics varied in the /{beta}/{delta} cell-type. No correlation was observed between the cell-types identity with time in culture or cell size. Clearly, multiple hormone transcripts can be expressed in islet cells whereas iGABAAR functional properties appear or {beta} cell specific.