bioRxivCancer Biology
LncRNA MIR4435-2HG mediates cisplatin resistance in HCT116 cells by regulating Nrf2 and HO-1
X. Zhong, P. Luo, S. Wu, C. Zhou, X. Yuan, H. Li, J. Chen, Y. Tian, Y. Qiu, ShuGui Wu,ChaoMing Zhou,Xia Yuan,HongMi Li,JinPing Chen,YunFei Tian,Yang Qiu,XiaoMing Zhong, Ping Luo
Abstract
PURPOSECisplatin resistance is still a serious problem in clinic. However, the underlying mechanism remains unclear. In this study investigated the drug resistance of cisplatin by the cisplatin resistance cell line HCT116R.\n\nRESULTSIn this study, we found that LncRNA MIR4435-2HG level dramatically increased in the cisplatin resistance cell line HCT116R. Knockdown of MIR4435-2HG in HCR116R significantly restored the sensitivity to cisplatin, inhibited cell proliferation and promoted cell apoptosis. Furthermore, Nrf2 and HO-1 mRNA level, as the critical molecular of the oxidative stress pathway, was inhibited by the siRNA targeting to MIR4435-2HG, displaying MIR4435-2HG-mediated cisplatin resistance through the Nrf2/HO-1 pathway.\n\nCONCLUSIONOur findings demonstrated that LncRNA MIR4435-2HG as a main factor could drive the cisplatin resistance of HCT116.
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Bypassing cisplatin resistance in Nrf2 hyperactivated head and neck cancer through effective PI3Kinase targeting
Pedram Yadollahi, Kelli A. McCord, … Vlad Constantin Sandulache·British Journal of CancerPeer-reviewed·Oct 6, 2025
- Targeted suppression of SPP1 inhibits tumor invasion and metastasis in NRF2 hyperactivated cisplatin resistant HNSCC
Mutsuki Kawabe, Sujuan Yang, … Abdullah A. Osman·Journal of Translational MedicinePeer-reviewed·May 22, 2026
- HMGB1 assists in overcoming cisplatin resistance in chemoresistant human ovarian cancer cells
Van Huynh, Guliang Wang, … Karen M. Vásquez·Molecular CarcinogenesisPeer-reviewed·Jan 9, 2026
- The critical role of HDAC1 activates NSCLC growth by nicotine resistance Cisplatin
Ching-Yi Peng, Jia‐Ping Wu·bioRxiv·Jun 4, 2020
- Cisplatin-inducible death receptor 5 antisense long non-coding RNA modulates cell cycle and proliferation in HeLa cells
D. C. Gurer, I. Erdogan, … B. Akgul·bioRxiv·Dec 23, 2020
- Close homologue of L1 sensitizes lung cancer cells to cisplatin and paclitaxel via inhibition Akt pathway