R. Huang

Shrimp Hemocyanin elicits a potent humoral response in mammals and is favorable to hapten conjugation

This study shows shrimp hemocyanin (SHC) can replace keyhole limpet hemocyanin (KLH) as a carrier protein for hapten conjugation, offering better solubility and comparable immunogenicity in mammals.

Riluzole reverses blood-testis barrier loss to rescue chemotherapy induced male infertility by binding to TRPC

This study shows riluzole restores blood-testis barrier function and rescues chemotherapy-induced male infertility in mice by targeting TRPC5.

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

This paper determines the 3D structures of SL5 RNA domains from six coronaviruses, revealing conserved tertiary features with implications for protein-binding and antiviral targets.

Ribonanza: deep learning of RNA structure through dual crowdsourcing

The paper introduces RibonanzaNet, a deep learning model trained on crowdsourced RNA chemical mapping data to predict RNA structure and related properties.

Genome-wide CRISPR screen identifies AC9 as a key regulator of ER calcium homeostasis involved in neuronal differentiation.

The paper identifies AC9 as a key regulator of ER calcium homeostasis during neuronal differentiation using CRISPR screening and functional analysis.

Systematic benchmarking of imaging spatial transcriptomics platforms in FFPE tissues

This study benchmarks three imaging spatial transcriptomics platforms on FFPE tissues, finding 10x Xenium has higher transcript counts without sacrificing specificity, while all platforms align with RNA-seq data but vary in performance metrics.

ONECUT2 Activates Diverse Resistance Drivers of Androgen Receptor-Independent Heterogeneity in Prostate Cancer

This study identifies ONECUT2 as a master transcription factor that drives resistance in prostate cancer through multiple mechanisms, including activation of neuroendocrine and adenocarcinoma variants, and shows that its inhibition can suppress treatment-resistant lineage plasticity.

PRO-LDM: Protein Sequence Generation with a Conditional Latent Diffusion Model

PRO-LDM generates protein sequences with tailored properties using a conditional latent diffusion model, enhancing diversity and design fidelity.

Local CpG-Stat3 siRNA treatment improves antitumor effects of checkpoint inhibitors

This study shows that local CpG-Stat3 siRNA treatment enhances checkpoint inhibitor efficacy in mouse tumor models by improving T-cell responses.

FLECS Technology for High-Throughput Screening of Hypercontractile Cellular Phenotypes in Fibrosis: A Function-First Approach to Anti-Fibrotic Drug Discovery

This study develops a high-throughput screening platform to identify anti-fibrotic compounds by quantifying myofibroblast contractility in response to TGF-β1.

Structure-Activity Relationship Study Identifies a Novel Lipophilic Amiloride Derivative that Efficiently Kills Chemoresistant Breast Cancer Cells

This study identifies a novel amiloride derivative, LLC1, that shows potent cytotoxicity against chemoresistant breast cancer cell lines but limited efficacy in a metastatic mouse model.

Self-regulation of PIN1-driven auxin transport by cell surface-based auxin signaling in Arabidopsis

The paper identifies a cell surface-triggered auxin signaling mechanism that regulates PIN1-mediated auxin efflux and developmental patterning in Arabidopsis.

ABLs and TMKs are co-receptors for extracellular auxin

The paper identifies ABL1 and ABL2 as new auxin receptors that function with TMKs as co-receptors for extracellular auxin, overlapping with but distinct from ABP1.

Dysregulation of Acid Ceramidase-mediated Sphingolipid Metabolism Contributes to Tumor Progression in Tuberous Sclerosis Complex

This study identifies dysregulated sphingolipid metabolism in TSC2-null cells and tumors, showing that ASAH1 inhibition reduces cell viability and tumor growth in TSC and LAM.

Quantitative bioactivity signatures of dietary supplements and natural products

The paper screens dietary supplements and natural products for bioactivity using in vitro assays and predicts their mechanisms of action by comparing activity profiles with approved drugs.

In vivo quantitative high-throughput screening for drug discovery and comparative toxicology

This paper describes an in vivo qHTS method using C. elegans to assess drug toxicity and therapeutic potential, demonstrating its feasibility with anti-infective agents and toxicological compounds.

RNA structure landscape of S. cerevisiae introns

The paper characterizes RNA structures in S. cerevisiae introns using DMS probing and identifies new structural elements that influence intron retention.

Cancer Cells in all EMT States Lack Rigidity Sensing Depletion of Different Tumor Suppressors Causes Loss of Rigidity Sensing in Cancer Cells

The paper shows that cancer cells across different EMT states lack rigidity sensing, often due to missing tumor suppressors like Tpm2.1, and this loss contributes to their transformed phenotype.

A toxin-mediated policing system in Bacillus improves population fitness via penalizing non-cooperating phenotypic cheaters

This study identifies a toxin-mediated policing system in Bacillus that punishes phenotypic cheaters, enhancing population fitness in plant rhizosphere and under stress.

Structures of the T cell potassium channel Kv1.3 with immunoglobulin modulators

This study determines the structures of the T cell potassium channel Kv1.3 with immunoglobulin modulators, revealing two distinct mechanisms to suppress channel activity.

Lifting the ban on nuclear import activates Gdown1-mediated modulation of global transcription and facilitates adaptation to cellular stresses

This study shows that nuclear import of Gdown1 modulates global transcription and enhances cellular stress tolerance.

Reference nodule transcriptomes for Melilotus officinalis and Medicago sativa cv. Algonquin

The paper reports nodule transcriptome assemblies for two legumes, identifying differences in NCR peptide profiles that may explain symbiotic compatibility differences with rhizobia.

Hsa-miR-31-5p controls a metabolic switch in psoriatic keratinocytes that identifies therapeutic intervention

This study shows that miR-31-5p in psoriatic keratinocytes promotes a metabolic switch to glutamine metabolism, leading to immune-modulatory effects and Th17 cell differentiation, which can be inhibited by GLS targeting.

Effects of Oncohistone Mutations and PTM Crosstalk on the N-terminal Acetylation Activities of NatD

This study investigates how oncohistone mutations and PTMs affect NatD-catalyzed N-terminal acetylation on histone H4/H2A, revealing their regulatory impacts.

A unique genomic island-governed cannibalism in Bacillus enhanced biofilm formation through a novel regulation mechanism

This study identifies a novel cannibalism mechanism in Bacillus velezensis SQR9 mediated by bacillunoic acids, enhancing biofilm formation and interspecies competition through a two-component regulatory system.

The cause of on-target point mutations generated by CRISPR-Cas9 treatment in the yeast Xanthophyllomyces dendrorhous

This study identifies on-target point mutations caused by CRISPR-Cas9 in yeast, showing sequence dependence and involvement of NHEJ components.

OPT3 Transports Copper to the Phloem, Mediates Shoot-to-Root Copper Signaling andCrosstalk Between Copper and Iron Homeostasis in A. thaliana

The paper identifies AtOPT3 as a phloem transporter that moves copper and iron, mediating shoot-to-root signaling and crosstalk between copper and iron homeostasis in A. thaliana.

Chemoproteomic study uncovers HemK2/KMT9 as a new target for NTMT1 bisubstrate inhibitors

The paper identifies HemK2/KMT9 as a new target of NTMT1 bisubstrate inhibitor NAH-C3-GPKK through chemoproteomic profiling.

Novel bisubstrate inhibitors for protein N-terminal acetyltransferase D

This study designs and characterizes potent bisubstrate inhibitors for NatD, showing high selectivity and revealing the enzyme's ordered Bi-Bi mechanism through crystal structures.

Development of a continuous fluorescence-based assay for N-terminal acetyltransferase D

The paper develops a fluorescence-based assay for NatD to identify small-molecule inhibitors, finding two hits from a compound screen.