Dynamic RNA 3' uridylation and guanylation during mitosis

ABSTRACTThe successful cell division involves highly regulated transcriptional and post-transcriptional control. Some of the cell cycle related genes are periodically expressed, while most of the genes show relatively stable steady state transcript level throughout the mitotic cell cycle (Bertoli et al., 2013; Park et al., 2016). Previous TAIL-seq analysis of S phase and M phase poly(A) tail information showed that less than 2% genes showed more than 2-fold change in their poly(A) tail length (Chang et al., 2014; Park et al., 2016). In addition, the changes in poly(A) tail length between these two stages showed minimal impact on the translation of the genes as long as the poly(A) tails were longer than 20 nt. Therefore, the significance of poly(A) tail dynamics during the cell cycle remains unknown. Here, by re-analyzing the S phase and M phase TAIL-seq data, we uncovered an interesting global dynamics of RNA poly(A) tails in terms of their terminal modifications, implying global RNA regulation between mitotic cell cycles through poly(A) tail terminal modifications.Competing Interest StatementThe authors have declared no competing interest.View Full Text