KsgA facilitates ribosomal small subunit maturation by proofreading a key structural lesion

Abstract

Ribosome assembly is orchestrated by many assembly factors, including ribosomal RNA methyltransferases whose precise role is poorly understood. Here, we leverage the power of cryo-EM and machine learning to discover that the bacterial methyltransferase KsgA performs a novel "proofreading" function in assembly of the ribosomal small subunit by recognizing and partially disassembling particles that have matured but are not competent for translation. We propose that this activity allows inactive particles an opportunity to reassemble into an active state, thereby increasing overall assembly fidelity. Detailed structural quantifications in our datasets additionally enabled expansion of the Nomura assembly map to highlight rRNA helix and r-protein interdependencies, which newly details how binding and docking of these elements are tightly coupled. These results have wide-ranging implications in our understanding of the quality control mechanisms governing ribosome biogenesis, and showcase the power of heterogeneity analysis in cryo-EM to unveil functionally relevant information in biological systems.