Joaquı́n Ortega

Non-canonical ribosome organization enables translation stalling in neuronal RNA granules.

The paper investigates ribosome clusters in neuronal RNA granules, finding they contain stalled ribosomes and unique 3D structures that avoid quality-control mechanisms.

FMRP Regulates Neuronal RNA Granules Containing Stalled Ribosomes, Not Where Ribosomes Stall

The paper investigates the role of FMRP in neuronal RNA granules containing stalled ribosomes and finds that its loss affects granule stability but not ribosome stalling sites.

Puromycin reveals a distinct conformation of neuronal ribosomes

The paper shows that puromycylated peptides remain in neuronal ribosomes in a hybrid state, revealing a distinct conformation and stalling mechanism in neurons.

Ribosomes in RNA granules are stalled on mRNA sequences that are consensus sites for FMRP association.

The paper identifies mRNA sequences that stall ribosomes in neurons, linking these sequences to FMRP association and stalled polysome function.

Alternative Conformations and Motions Adopted by 30S Ribosomal Subunits Visualized by Cryo-Electron Microscopy.

This study uses cryo-electron microscopy to determine the structure of the inactive conformation of the 30S ribosomal subunit and analyze its motions and structural changes under different conditions.

Role of Era in Assembly and Homeostasis of the Ribosomal Small Subunit

This study investigates Era's role in 30S ribosomal subunit assembly using mass spectrometry, cryo-EM, and in-cell footprinting, showing that most rRNA domains can fold without Era and that Era binding affects the decoding center.