bioRxivPharmacology & ToxicologyPreprint
Human embryonic stem cell-derived cardiomyocytes express SARS-CoV-2 host entry proteins: screen to identify inhibitors of infection
T. L. Williams, M. T. Colzani, R. G. Macrae, E. L. Robinson, S. Bloor, E. J. Greenwood, J. R. Zhan, G. Strachan, R. E. Kuc, D. Nyimanu, J. J. Maguire, P. J. Lehner, S. Sinha, A. P. Davenport
Abstract
Patients with cardiovascular comorbidities are more susceptible to severe infection with SARS-CoV-2, known to directly cause pathological damage to cardiovascular tissue. We outline a screening platform using human embryonic stem cell-derived cardiomyocytes, confirmed to express the protein machinery critical for SARS-CoV-2 infection, and a pseudotyped virus system. The method has allowed us to identify benztropine and DX600 as novel inhibitors of SARS-CoV-2 infection.
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Human iPSC-Derived Cardiomyocytes are Susceptible to SARS-CoV-2 Infection
A. Sharma, G. Garcia, … C. N. Svendsen·bioRxiv·Apr 21, 2020
- SARS-CoV-2 infects and induces cytotoxic effects in human cardiomyocytes
D. Bojkova, J. Wagner, … S. Dimmeler·bioRxiv·Jun 1, 2020
- SARS-CoV-2 Infects Human Engineered Heart Tissues and Models COVID-19 Myocarditis
A. L. Bailey, O. Dmytrenko, … K. J. Lavine·bioRxiv·Nov 4, 2020
- SARS-CoV-2 infects human pluripotent stem cell-derived cardiomyocytes, impairing electrical and mechanical function
S. Marchiano, T.-Y. Hsiang, … C. E. Murry·bioRxiv·Aug 30, 2020
- Highly efficient SARS-CoV-2 infection of human cardiomyocytes: spike protein-mediated cell fusion and its inhibition
C. K. Navaratnarajah, D. R. Pease, … Boyd Rasmussen and Frank J. Secreto, Timothy J. Nelson (Director)·bioRxiv·Jul 30, 2021
- WIN 55,212-2 shows anti-inflammatory and survival properties in human iPSC-derived cardiomyocytes infected with SARS-CoV-2
L. G. H. S. Aragao, J. T. Oliveira, … S. Rehen·bioRxiv·Feb 20, 2021