bioRxivCancer BiologyPreprint
Modelling cancer immunomodulation using epithelial organoid cultures
Yotam E. Bar-EphraimiD, Kai KretzschmariD, Priyanca Asra, Evelien de Jongh, Kim E. Boonekamp, Jarno DrostiD, Joost van Gorp, Apollo Pronk, Niels Smakman, Inez J. Gan, Zsolt SebestyéniD, Jürgen KuballiD, Robert G.J. Vries, Hans CleversiD
Abstract
Here we utilize organoid technology to study immune-cancer interactions and assess immunomodulation by colorectal cancer (CRC). Transcriptional profiling and flow cytometry revealed that organoids maintain differential expression of immunomodulatory molecules present in primary tumours. Finally, we established a method to model antigen-specific epithelial cell killing and cancer immunomodulation in vitro using CRC organoids co-cultured with cytotoxic T cells (CTLs).
- organism: human
- assay: organoid / primary culture
- research focus: cancer & tumor microenvironment
- approach: experimental assay
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