Analysis of allelic series with transcriptomic phenotypes

Although transcriptomes have recently been used to perform epistasis analyses, they are not yet used to study intragenic function/structure relationships. We developed a theoretical framework to study allelic series using transcriptomic phenotypes. As a proof-of-concept, we apply our methods to an allelic series of dpy-22, a highly pleiotropic Caenorhabditis elegans gene orthologous to the human gene MED12, which is a subunit of the Mediator complex. Our methods identify functional regions within dpy-22 that modulate Mediator activity upon various genetic modules.