Molecular Systems BiologySystems BiologyPeer-reviewed journal article
In Situ Genotyping Of A Pooled Strain Library After Characterizing Complex Phenotypes
Michael J. LawsoniD, Daniel CamsundiD, Jimmy LarssoniD, Özden BaltekiniD, David FangeiD, Johan ElfiD
Abstract
So far, it has not been possible to perform advanced microscopy on pool generated strain libraries and at the same time know each strains genotype. We have overcome this barrier by identifying the genotypes for individual cells in situ after a detailed characterization of the phenotype. The principle is demonstrated by single molecule fluorescence imaging of E. coli strains harboring barcoded plasmids that express a sgRNA which suppress different genes through dCas9.
- Citations
- 83
- FWCI
- 2.90
- Field percentile
- top 8%
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Pooled optical screening in bacteria using chromosomally expressed barcodes
R. R. G. Soares, D. A. Garcia-Soriano, … J. Elf·Communications BiologyPeer-reviewed·Jun 3, 2025
- High-throughput, image-based screening of genetic variant libraries
George Emanuel, Jeffrey R. Moffitt, Xiaowei Zhuang·Nature MethodsPeer-reviewed·Oct 30, 2017
- Optical pooled screening of a bacterial transposon mutagenesis library
Oscar Broström, Praneeth Karempudi, … Johan Elf·bioRxiv·Jul 7, 2026
- Real-time pooled optical screening with single-cell isolation capability.
P. Karempudi, E. Amselem, … J. Elf·bioRxiv·Sep 21, 2023
- Highly replicated experiments studying complex genotypes using nested DNA barcodes.
Molly Monge, Simone M. Giovanetti, … Meru J. Sadhu·G3 Genes Genomes GeneticsPeer-reviewed·Jul 14, 2025
- Linking live-cell imaging to single-cell sequencing using genetic barcoding
Micha Muller, Silke J. A. Lochs, … Marvin E. Tanenbaum·bioRxiv·Oct 8, 2026