Advanced biosensing and bioanalysis techniques

Microbead-Based Heterodimer Assay for Amplification-Free Detection of Single-Stranded DNA

This paper introduces a microbead-based heterodimer assay for direct, amplification-free detection of single-stranded DNA targets at low concentrations.

Topographical features enhance DNA tetrahedron biostability

This study shows that adding hairpins to DNA tetrahedra increases their nuclease resistance, enhancing biostability through sequence-specific stabilization.

Magnetic molecular nanorulers enable combinatorial biosensing and determine distances at the molecular level

This paper introduces MARs, magnetic molecular nanorulers that measure molecular distances and enable combinatorial biosensing with high accuracy and resolution.

AutoMod: A Software Tool for High-Accuracy Design of Curved DNA Origami Nanostructures

AutoMod improves DNA origami design accuracy by reducing shape deviation in curved structures through semi-automated sequence generation and benchmarking.

Selection of DNA aptamers targeting PirAB toxin for potential biosensing applications in acute hepatopancreatic necrosis disease (AHPND)

This paper optimizes SELEX to develop DNA aptamers targeting PirAB toxin for early detection of AHPND, achieving Kd values of 65-700 nM for PirB and 1-3 µM for PirAB complex.

High-throughput variant libraries and machine learning enable robust target-site selection for toehold-switch design in Detectrons

DeSTAR is a machine learning model that ranks target sites for RNA sensors, improving performance across different transcripts.

A modular DNA toolkit for single-molecule biophysics

The paper introduces a modular DNA toolkit for creating long, customizable DNA molecules with user-defined sequences for single-molecule biophysics applications.

Rapid Isothermal Detection of Heavy Metals via Transcription-Factor-Gated DNA Strand Synthesis

This paper introduces TONE, a rapid, isothermal biosensing platform for heavy metal detection using transcription-factor-gated DNA strand-displacement amplification, achieving low detection limits for copper and lead.