Anaerobic Digestion and Biogas Production

Lactate:propionate molar ratio and substrate concentrations shape valerate production in secondary lactate fermentations

This study investigates how lactate:propionate ratios and substrate concentrations affect valerate production in continuous lactate fermentations.

Methanogenic Ethanol Production from Acetate

This study shows Methanosarcina acetivorans can convert acetate to ethanol and methane, producing 40% ethanol from acetate, more than from methane or with a modified Mcr.

Reduced carbon emissions and chain elongation during mixotrophic fermentation of a biomass feedstock

This study tests mixotrophic fermentation of biomass and syngas, finding reduced CO2 emissions and increased production of C4 and C6 carboxylates.

digestome: a licence-clean marker-gene panel for functional profiling of anaerobic digestion microbiomes

The paper introduces digestome, a marker-gene panel for functional profiling of anaerobic digestion microbiomes, and benchmarks it against metagenome-assembled genomes and species representatives.

Deciphering efficient metabolic pathways in an anaerobic gut fungus coupled with a syntrophic methanogen

This study investigates metabolic pathways in anaerobic gut fungi and their syntrophic methanogen partners using 13C-glucose and supplements, revealing key metabolic contributions and growth effects.

Scaling down-Evaluation of start-up and microbial stabilisation dynamics in five parallel laboratory-scale CSTR biogas system

This study evaluates start-up and stabilisation dynamics in five parallel CSTR biogas systems, finding that microbial communities diverge from the original inoculum and require extended stabilisation.

Coupling Stickland fermentation with reverse β-oxidation represents a new mode of medium-chain carboxylate production

This study identifies a novel metabolic pathway in chain elongating bacteria that couples Stickland fermentation with reverse β-oxidation to produce medium-chain carboxylic acids.

Comparative analysis of adhesin-like proteins from Methanobacteriales species

The paper analyzes adhesin-like proteins (ALPs) in Methanobacteriales species to understand their role in gut microbiota interactions and species-specific adaptations.

Redirecting carbon and electron flow in methanogenic laboratory scale anaerobic digestors with hypophosphite

This study shows that hypophosphite inhibits formate exchange in anaerobic digestors, reducing methane production and redirecting carbon and electron flow to hydrogen and volatile fatty acids without altering microbiome composition.

Increased substrate complexity drives re-diversification and functional reorganization in simplified methanogenic consortia

This study shows that adding complex substrates to simplified methanogenic communities can restore diversity but may reduce process stability and methane production.

Peptonella octanoica gen. nov., sp. nov., a new medium-chain carboxylate-producing bacterium, and the reclassification of Eubacterium pyruvativorans as Peptonella pyruvativorans comb. nov.

The paper describes a new bacterial species, Peptonella octanoica, and reclassifies Eubacterium pyruvativorans into this genus based on phylogenetic and phenotypic analysis.

LANTHANUM (LaCl3) ADDITION DIVERSIFIES ORGANIC ACID PRODUCTION AND SIGNIFICANTLY ENHANCES METHANE PRODUCTION IN A METHANOGENIC CONSORTIUM

This study shows that adding lanthanum chloride (LaCl3) increases biogas and methane production in a methanogenic consortium, with the highest yields at 100 mg/L.

Long-term Production and Recovery of Medium-Chain Carboxylates from Source-Separated Organics

This study demonstrates long-term production and recovery of medium-chain carboxylic acids from source-separated organics using microbial chain elongation and in-line extraction.

Modelling Anaerobic Co-Digestion with Agricultural Feedstock: Model Validation and Cross-Reactor Verification

This paper validates a mass-based ADM1 model for anaerobic co-digestion of maize silage and cow manure, showing high predictive accuracy with R2 values of 0.81 and 0.85 in calibration and validation tests.

Towards complete carbon utilization: Improved methane yield from formate and hydrogen co-feeding through constitutive formate dehydrogenase-gene expression in Methanothermobacter thermautotrophicus ΔH

This study improves methane yield from formate and hydrogen co-feeding by engineering a methanogen to bypass hydrogen-induced genetic regulation of formate metabolism.

Carbon monoxide utilisation by Thermanaeromonas species and description of Thermobium azorense gen. nov., sp. nov.

This study isolates a novel thermophilic CO-utilising bacterium, describes its genomic potential for CO utilisation, and proposes a new genus and species.

Uncovering microbial life-history strategies under disturbance: a trait-based computational analysis of anaerobic systems

The paper applies a trait-based framework to anaerobic microbial communities under disturbance, identifying three life-history strategies.

Mass-transfer-constrained thermodynamics links fluid motion to the preferential use of hydrogen and formate in syntrophic propionate oxidation

The paper shows fluid motion affects electron-transfer pathway selection in syntrophic propionate oxidation by altering thermodynamic favorability of hydrogen and formate.

Minimizing methane emissions during the degradation of sewage sludge in a sulfate-rich bioreactor

This study shows that sulfate-rich bioreactors can reduce methane emissions during sewage sludge degradation by manipulating microbial communities and organic load ratios.

Organic availability and microbial competition for acetate suppress methane emissions during the conversion of gypsum in sewage sludge

This study shows that microbial community composition and organic availability influence methane emissions during gypsum conversion in sewage sludge, with sulfate-reducing bacteria suppressing methane production.

Hypophosphite is a naturally-occurring selective inhibitor of syntrophic methanogenesis

This study identifies hypophosphite as a natural inhibitor of syntrophic methanogenesis, offering a potential strategy for methane control in ecosystems.

Dairy wastewater grease stabilizes in situ mesophilic biomethanation for H2-to-CH4 conversion

This paper demonstrates a stable mesophilic biomethanation process using dairy wastewater grease that converts H2 to CH4 efficiently without continuous H2 supply or pressurization.

Methanobacterium nebraskense sp. nov., a hydrogenotrophic methanogen isolated from saline wetland soil

This paper describes the isolation and characterization of a new methanogenic species, Methanobacterium nebraskense, from saline wetland soil.

Thermoaminiphila catenidiffluenda gen. nov., sp. nov.: A novel thermophilic, strictly anaerobic bacterium representing Thermoaminiphilia class nov., a newly described class thriving in hydrocarbon-rich habitats and biogas fermenters

The paper describes a novel thermophilic, strictly anaerobic bacterium isolated from biogas fermenters and hydrocarbon-rich habitats, expanding the taxonomic diversity of the Bacillota phylum.

Distinct Acetate Utilization Strategies Differentiate Butyrate and Medium-Chain Carboxylic Acid Producing Chain-Elongating Bacteria

The paper identifies distinct acetate utilization strategies in chain-elongating bacteria that explain differences in butyrate versus medium-chain carboxylic acid production.

Comparing the performance of functional versus taxonomic metagenomics for detecting ammonia disturbances in the biogas system

This study compares functional and taxonomic metagenomics for detecting ammonia disturbances in biogas systems and finds taxonomic data performs as well or better than functional data.

Dried-bakery waste as a substrate for n-caproate and n-caprylate production via chain elongation: bakeroate

This study converts bakery waste into n-caproate and n-caprylate using microbial chain elongation, achieving 30-35% carbon conversion with lactate as a superior intermediate.

CAZyme domain architectures suggest fine-scale functional differentiation among anaerobic fungi and bacteria during lignocellulose conversion to volatile fatty acids

Enhanced Resistance and Resilience of Anaerobic Digestion Microbiome after Single and Dual Short-Term Disturbances

This study shows that short-term pulse disturbances improve anaerobic digestion microbiome resilience, enabling faster recovery from overloading and stable performance at shorter solids retention times.

Bioaugmentation in anaerobic digesters: A systematic review

This review evaluates bioaugmentation strategies in anaerobic digesters, finding that most studies focus on hydrolysis and dark fermentation, with limited research on acetogenesis and syntrophic oxidation.