Anaerobic Digestion and Biogas Production

Methanogenic Ethanol Production from Acetate

This study shows Methanosarcina acetivorans can convert acetate to ethanol and methane, producing 40% ethanol from acetate, more than from methane or with a modified Mcr.

digestome: a licence-clean marker-gene panel for functional profiling of anaerobic digestion microbiomes

The paper introduces digestome, a marker-gene panel for functional profiling of anaerobic digestion microbiomes, and benchmarks it against metagenome-assembled genomes and species representatives.

Deciphering efficient metabolic pathways in an anaerobic gut fungus coupled with a syntrophic methanogen

This study investigates metabolic pathways in anaerobic gut fungi and their syntrophic methanogen partners using 13C-glucose and supplements, revealing key metabolic contributions and growth effects.

Coupling Stickland fermentation with reverse β-oxidation represents a new mode of medium-chain carboxylate production

This study identifies a novel metabolic pathway in chain elongating bacteria that couples Stickland fermentation with reverse β-oxidation to produce medium-chain carboxylic acids.

Redirecting carbon and electron flow in methanogenic laboratory scale anaerobic digestors with hypophosphite

This study shows that hypophosphite inhibits formate exchange in anaerobic digestors, reducing methane production and redirecting carbon and electron flow to hydrogen and volatile fatty acids without altering microbiome composition.

Increased substrate complexity drives re-diversification and functional reorganization in simplified methanogenic consortia

This study shows that adding complex substrates to simplified methanogenic communities can restore diversity but may reduce process stability and methane production.

Peptonella octanoica gen. nov., sp. nov., a new medium-chain carboxylate-producing bacterium, and the reclassification of Eubacterium pyruvativorans as Peptonella pyruvativorans comb. nov.

The paper describes a new bacterial species, Peptonella octanoica, and reclassifies Eubacterium pyruvativorans into this genus based on phylogenetic and phenotypic analysis.

LANTHANUM (LaCl3) ADDITION DIVERSIFIES ORGANIC ACID PRODUCTION AND SIGNIFICANTLY ENHANCES METHANE PRODUCTION IN A METHANOGENIC CONSORTIUM

This study shows that adding lanthanum chloride (LaCl3) increases biogas and methane production in a methanogenic consortium, with the highest yields at 100 mg/L.

Carbon monoxide utilisation by Thermanaeromonas species and description of Thermobium azorense gen. nov., sp. nov.

This study isolates a novel thermophilic CO-utilising bacterium, describes its genomic potential for CO utilisation, and proposes a new genus and species.

Mass-transfer-constrained thermodynamics links fluid motion to the preferential use of hydrogen and formate in syntrophic propionate oxidation

The paper shows fluid motion affects electron-transfer pathway selection in syntrophic propionate oxidation by altering thermodynamic favorability of hydrogen and formate.

Minimizing methane emissions during the degradation of sewage sludge in a sulfate-rich bioreactor

This study shows that sulfate-rich bioreactors can reduce methane emissions during sewage sludge degradation by manipulating microbial communities and organic load ratios.

Organic availability and microbial competition for acetate suppress methane emissions during the conversion of gypsum in sewage sludge

This study shows that microbial community composition and organic availability influence methane emissions during gypsum conversion in sewage sludge, with sulfate-reducing bacteria suppressing methane production.

Dairy wastewater grease stabilizes in situ mesophilic biomethanation for H2-to-CH4 conversion

This paper demonstrates a stable mesophilic biomethanation process using dairy wastewater grease that converts H2 to CH4 efficiently without continuous H2 supply or pressurization.

Thermoaminiphila catenidiffluenda gen. nov., sp. nov.: A novel thermophilic, strictly anaerobic bacterium representing Thermoaminiphilia class nov., a newly described class thriving in hydrocarbon-rich habitats and biogas fermenters

The paper describes a novel thermophilic, strictly anaerobic bacterium isolated from biogas fermenters and hydrocarbon-rich habitats, expanding the taxonomic diversity of the Bacillota phylum.

CAZyme domain architectures suggest fine-scale functional differentiation among anaerobic fungi and bacteria during lignocellulose conversion to volatile fatty acids

Elucidating redox-driven inhibition of methanogenesis by an artificial quinone in Methanosarcina barkeri: Integrated proteomic and physiological evidence

This study investigates how an artificial quinone inhibits methanogenesis in M. barkeri by analyzing physiological and proteomic responses to 2,7-AQDS.

Enhanced Hydrogen Production Through Two-Stage Fermentation Coupling Clostridium pasteurianum and Rhodobacter sphaeroides

This paper develops a two-stage fermentation system using Clostridium pasteurianum and Rhodobacter sphaeroides to enhance hydrogen production while reducing inhibitory metabolite accumulation.

Phylum-wide propionate degradation and its potential connection to poly- γ-glutamate biosynthesis in Candidatus Cloacimonadota

This study identifies an alternative methylmalonyl-CoA pathway in Cloacimonadota, linking propionate degradation to poly-γ-glutamate biosynthesis and syntrophic interactions with methanogens.

Enhancing Volatile Fatty Acid Accumulation in Seaweed-Arrested Anaerobic Digestion via a Two-Tier Framework of Engineering Diagnostics and Microbial Surveillance

Variations in H2 thresholds and growth yields reveal bioenergetic diversity among hydrogenotrophic methanogens

This study measures H2 thresholds and growth yields of nine hydrogenotrophic methanogens, revealing significant variations in their bioenergetic strategies and environmental relevance.

Micro-aeration-enhanced Anaerobic Digestion for the Stabilization of Coffee-Processing Wastewater

This study evaluates micro-aeration-enhanced anaerobic digestion for treating coffee-processing wastewater, finding it improves recalcitrant compound removal but reduces methane yield.

Structure of the energy converting methyltransferase (Mtr) of Methanosarcina mazei in complex with an oxygen-stress responsive small protein

The paper presents a cryo-EM structure of the Mtr complex from Methanosarcina mazei and identifies a small ORF protein, MtrI, involved in oxygen stress response.

H2 dependent modulation of Mtr activity by small protein MtrR in Methanosarcina mazei

The paper characterizes the small protein MtrR in Methanosarcina mazei, showing it modulates Mtr activity in response to hydrogen, affecting growth under varying H2 conditions.

Propionic acid-related inhibition during anaerobic digestion: insights into methane production and microbial community adaptation

This study investigates how propionic acid affects anaerobic digestion by comparing its effects to sodium propionate and NaCl, showing acidity is the main inhibitor.

Sludge liquefaction and stratification enable ultra-high digestion rate and resource recovery for waste activated sludge treatment

The paper introduces a novel sludge treatment process called SPREAD that significantly improves organic loading rates and nutrient recovery in waste activated sludge treatment.

Microbiome-oriented data mining of operational monitoring of anaerobic digestion reactor during steady operation period, failure, and restoration.

This study uses microbiome data mining to analyze microbial shifts in anaerobic digestion reactors during operation, failure, and restoration, identifying key taxa and operational parameters linked to stability.

The effect of feeding on microbiome and biogas composition in anaerobic CSTR

This study examines how different substrates affect biogas yield and microbial dynamics in anaerobic CSTRs, finding that a protein-rich substrate increases biogas production and methane content.

Steering the chain-elongating microbiome to specific medium-chain carboxylates with ethanol and lactate as co-electron donors: maximizing C8 or C6

This study identifies operational conditions to steer microbiomes toward producing specific medium-chain carboxylates, finding that ethanol-to-lactate ratios and temperature influence product specificity.

Physiological barriers for glucose utilization in Methanosarcina acetivorans

This paper investigates why Methanosarcina acetivorans cannot use glucose for methanogenesis despite having glycolytic and gluconeogenic pathways, finding that glucose causes growth inhibition and carbohydrate accumulation.

ADToolbox: Incorporating Metagenomics Data for Improved Prediction of Anaerobic Digestion Dynamics

ADToolbox integrates metagenomics data into an enhanced ADM model to improve prediction of anaerobic digestion dynamics and VFA production.