Discover, rate, and verify biology preprints.

Compact type II-D Cas9 nucleases for efficient and specific genome editing

The paper identifies and characterizes compact Cas9d orthologs with high genome editing efficiency and specificity, suitable for AAV-based therapeutic applications.

Proximity directs microbial transglutaminase site selectivity in native antibody modification

This paper demonstrates that fusion-protein architecture determines site selectivity in proximity-directed enzymatic modification of antibodies, enabling site-specific functionalization.

Triceps surae and Achilles tendon contributions to ankle stiffness depend on movement state

This study quantifies how triceps surae and Achilles tendon contribute to ankle stiffness during posture and movement, finding that muscle stiffness decreases during movement, leading to more equal contributions from both structures.

From video-derived feeding behaviour to cow-level nutritional deviation signals: A dairy digital-twin decision-support framework

This paper develops a digital-twin framework that integrates video-derived feeding behavior with physiological data to improve precision nutrition in dairy cows, showing reduced prediction errors in dry matter intake.

In vitro and in silico characterization of competitive inhibition and repression of DUX4 target gene activation as a therapeutic approach for facioscapulohumeral muscular dystrophy (FSHD)

This paper demonstrates that a DUX4 DNA-binding domain fused to a human KRAB domain can effectively repress DUX4-FL transcriptional activity in vitro, and develops computational models to explore its therapeutic potential for FSHD.

Dissolution-Controlled Nanocrystalline Rifapentine Formulation for Tuberculosis Treatment

This study develops dissolution-controlled nanocrystalline rifapentine formulations that maintain therapeutic concentrations for over a week, showing efficacy in TB prevention and treatment in mice.

Differentiation and maturation of iPSC-derived motor and sensory neurospheres using biomodified PEG-based microgels

This study examines the differentiation and maturation of motor and sensory neurospheres derived from iPSCs using bio-modified PEG microgels, showing subtype-specific responses and structural differences.

Ancient human mitochondrial genomes encode antimicrobial peptides

The paper identifies antimicrobial peptides encoded in ancient human mitochondrial genomes and validates their activity against bacteria.

Tumor-tropic E. coli engineered as living T and NK cell engagers

This paper develops LIME, a bacterial platform that engagers T and NK cells to target tumors, showing enhanced cytotoxicity and immune response in preclinical models.

Ultrasound-reconfigurable scaffolds enable dynamic control of biochemical and biophysical cues for vascular network formation

This study develops ultrasound-responsive scaffolds that dynamically control biochemical and biophysical cues to enhance vascular network formation in vitro and in vivo.

Compact type II-C Cas9 nucleases with expanded PAM access and high fidelity for therapeutic genome editing

The paper identifies and characterizes three compact type II-C Cas9 orthologs with improved PAM compatibility and high fidelity for genome editing in human cells.

Learning protein function through autonomous experimental interaction

This paper demonstrates an AI system that autonomously interacts with biological systems to discover new protein functions through continuous experimental feedback.

Constrained Laser-Induced Cavitation for Miniaturized Treatment of Deep Vein Thrombosis

This paper introduces CLIC, a novel method for thrombolysis that removes clots more efficiently than existing techniques, with a mass-loss rate 40-fold higher and reduced embolic risk.

Additive manufacturing of PEDOT:PSS electrodes on collagen substrates for soft and bioactive electronics

This paper develops a method to create bioelectronic interfaces using collagen and conducting polymers, enhancing biointegration and cell compatibility.

LIT (Layer-Wise Image Trajectories): In Situ Monitoring for Early Quality Prediction and Anomaly Detection in Acellular and Cell-Laden Two-Photon Polymerization

This paper introduces LIT, a method to predict and detect anomalies in 2PP fabrication quality in real-time, without needing post-processing labels.

Periodic DNA encoding enables error-tolerant multi-class molecular detection by nanopore sequencing

This paper introduces a method to detect multiple molecular classes using nanopore sequencing by encoding targets into periodic DNA barcodes, achieving high specificity and error tolerance.

Bioprinted Human Primary Arteries Recapitulate Inflammatory Activation and Pharmacologic Rescue

This study develops a bioprinted artery-on-a-chip model to study inflammatory responses and test drug efficacy in human arteries.

Sex Differences in Aortic Remodeling During Blood Pressure Reduction Following Gradual Hypertension Development in Spontaneously Hypertensive Rats

This study examines sex differences in aortic remodeling after antihypertensive treatment in rats with gradually developed hypertension, finding more effective reversal in females.

Engineering a pH-sensitive humanized infliximab with improved potency and developability using STEM™

This study humanizes and engineers infliximab to improve its potency and developability, identifying LW2Y as a lead candidate with enhanced pH-dependent properties.

Controlled Substrate Crossover from Cathode to Anode for Long-Term Autonomous Operation of Microbial Fuel Cells: A Transport-Reaction Modeling Study

This paper reframes substrate crossover in microbial fuel cells as a design variable to sustain microbial metabolism and improve long-term operation.