Author

A. M. Jacobi

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Bioengineering· 1 Cell Biology· 1 Immunology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:genetic perturbation / CRISPR· 2 Research Focus:cell type annotation· 1 Assay:CRISPR screen· 1 Research Focus:drug response / drug discovery· 1 Approach:experimental assay· 1 Research Focus:gene regulatory network inference· 1 Organism:human· 1 Organism:mouse· 1

Frequent Co-Authors

A. J. Heidersbach· 1 shared A. Kuenkele· 1 shared A. Pruss· 1 shared A. Rehm· 1 shared A. Tyrer· 1 shared A. von Kriegsheim· 1 shared A. Wilhelm· 1 shared B. Haley· 1 shared

Activity by Year

2018-2022 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 14 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

1 shared paperBioengineering

A. J. Heidersbach

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

A versatile, high-efficiency platform for CRISPR-based gene activation

A. J. Heidersbach, K. M. Dorighi, J. A. Gomez, A. M. Jacobi, B. Haley

This paper introduces a high-efficiency CRISPRa platform combining piggyBac transposon technology and optimized guide RNA scaffolds to enable rapid, stable gene activation in diverse cellular contexts.

bioRxiv · Jul 21, 2022Bioengineering

Fast, efficient and virus-free generation of TRAC-replaced CAR T cells

J. Kath, W. Du, B. Thommandru, R. Turk, L. Amini, M. Stein, T. Zittel, S. Martini, L. Ostendorf, A. Wilhelm, L. Akyuez, A. Rehm, U. E. Hoepken, A. Pruss, A. Kuenkele, , D. L. Wagner

This paper presents a virus-free method to generate CAR T cells by inserting CARs into the TRAC locus using CRISPR-Cas and HDR, achieving up to 68% TCR-to-CAR replacement.

bioRxiv · Feb 14, 2021Immunology

An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein

P. S. Dewari, B. Southgate, K. Mccarten, G. Monogarov, E. O'Duibhir, N. Quinn, A. Tyrer, C. Plumb, C. Blin, R. Finch, R. B. Bressan, G. Morrison, A. M. Jacobi, M. A. Behlke, A. von Kriegsheim, , S. M. Pollard

The paper introduces an efficient, selection-free, and scalable CRISPR/Cas9-based pipeline for epitope tagging in mammalian stem cells, achieving knock-in efficiencies of 5-30%.

bioRxiv · Jan 29, 2018Cell Biology