D. G. Drubin

Interdependence of a microtubule polymerase and a motor protein in establishment of kinetochore end-on attachments

This study shows that Kip3 and Stu2 work together in yeast to establish proper kinetochore-microtubule attachments during mitosis.

Kinetic investigation reveals an HIV-1 Nef-dependent increase in AP-2 recruitment and productivity at endocytic sites

The paper investigates how HIV-1 Nef enhances AP-2 recruitment and productivity at endocytic sites through clathrin-mediated endocytosis.

Endocytic myosin-1 is a force-insensitive, power-generating motor

The paper measures the force-sensitivity of yeast endocytic myosin Myo5 and finds it generates power rather than acting as a force-sensitive anchor during endocytosis.

Self-organizing actin networks drive sequential endocytic protein recruitment and vesicle release on synthetic lipid bilayers

The paper reconstitutes actin-driven vesicle release from lipid bilayers using purified yeast WASP and cytoplasmic extracts, revealing sequential protein recruitment and mechanical forces involved in endocytosis.

Reconstitution of Kinetochore and Microtubule Dynamics Reveals a Role for a Kinesin-8 in Establishing End-on Attachments

This study reconstitutes kinetochore-microtubule interactions in yeast to show that Kip3 enables directional kinetochore movement and end-on attachment by regulating microtubule dynamics.

Rescue of stalled clathrin-mediated endocytosis by asymmetric Arp2/3-mediated actin assembly

The paper investigates how asymmetric actin assembly rescues stalled clathrin-mediated endocytosis by analyzing endocytic dynamics and protein geometry using advanced imaging and computational methods.

Induced Membrane Curvature Bypasses Clathrin's Essential Endocytic Function

The paper shows induced membrane curvature can drive endocytosis without clathrin, but not AP2, indicating clathrin's role is in curvature formation, not protein recruitment.

Actin force generation in vesicle formation: mechanistic insights from cryo-electron tomography

The paper uses cryo-electron tomography to study actin filament organization during clathrin-mediated endocytosis, revealing how actin dynamics generate pulling forces for vesicle formation.

An organelle inheritance pathway during polarized cell growth

The paper tracks organelle inheritance during budding yeast mitosis and finds a preferred sequence that is not exclusively controlled by cell cycle signaling.

Spatial regulation of clathrin-mediated endocytosis through position-dependent site maturation

The paper quantitatively analyzes clathrin-mediated endocytosis in yeast, showing that cargo-sensitive site maturation, not initiation, regulates endocytic internalization.

Evolutionarily related small viral fusogens hijack distinct but modular actin nucleation pathways to drive cell-cell fusion

This study shows that two FAST proteins use distinct actin nucleation pathways to drive cell-cell fusion, but their cytoplasmic tails are modular and functionally interchangeable.

Load adaptation of endocytic actin networks

The paper investigates how actin networks adapt to membrane tension during clathrin-mediated endocytosis, finding that actin assembly increases under elevated load to maintain CME robustness.

Cdc42 GTPase regulates ESCRTs in nuclear envelope sealing and ER remodeling

The paper identifies Cdc42's role in regulating ESCRT proteins at the nuclear envelope and ER fission sites, impacting nuclear envelope sealing and ER remodeling.

Direct comparison of clathrin-mediated endocytosis in budding and fission yeast reveals conserved and evolvable features

This study compares clathrin-mediated endocytosis in budding and fission yeast, finding conserved mechanisms and species-specific adaptations in actin assembly and membrane invagination.

Principles of self-organization and load adaptation by the actin cytoskeleton during clathrin-mediated endocytosis

The paper uses a multiscale model to show that actin networks enable clathrin-mediated endocytosis by self-organizing and adapting to membrane tension through Arp2/3 complexes and bent filaments.

Simultaneous 3D super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for cell imaging

This paper introduces a combined SIM and AFM platform for simultaneous 3D super-resolution fluorescence and atomic force microscopy, enabling spatially correlated imaging of cells without cell-to-cell variation.

Design principles for robust vesiculation in clathrin-mediated endocytosis

The paper investigates the physical design principles that enable robust vesiculation in clathrin-mediated endocytosis under varying membrane tensions.