Author

D. H. Siepe

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 1 Bioengineering· 1 Cell Biology· 1

Signature Assays, Organisms & Methods

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Assay:proteomics· 3 Research Focus:cell type annotation· 2 Approach:experimental assay· 2 Organism:human· 2 Approach:biophysical modeling· 1 Research Focus:cell signaling & trafficking· 1 Research Focus:cell-cell communication· 1 Approach:deep learning· 1

Frequent Co-Authors

K. C. Garcia· 3 shared A. Inoue· 1 shared B. Barsi-Rhyne· 1 shared B. K. Kobilka· 1 shared F. M. Heydenreich· 1 shared G. T. Hess· 1 shared J. Janetzko· 1 shared K. Kawakami· 1 shared

Activity by Year

2021-2022 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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3 shared papersBioengineering

K. C. Garcia

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

Receptor Elimination by E3 Ubiquitin Ligase Recruitment (REULR): A Targeted Protein Degradation Toolbox

D. H. Siepe, L. K. Picton, K. C. Garcia

This paper introduces REULR, a nanobody-based degradation toolbox that enables targeted elimination of cell surface receptors by recruiting E3 ubiquitin ligases, demonstrating effective receptor clearance and modulation of signaling.

bioRxiv · Oct 31, 2022 · PreprintBioengineering

Identification of orphan ligand-receptor relationships using a cell-based CRISPRa enrichment screening platform.

D. H. Siepe, L. T. Henneberg, S. C. Wilson, G. T. Hess, M. C. Bassik, K. Zinn, K. C. Garcia

The paper develops a CRISPRa-based screening platform to identify orphan ligand-receptor interactions by screening secreted proteins against cell surface receptor libraries.

bioRxiv · Jun 22, 2022Cell Biology

Membrane phosphoinositides stabilize GPCR-arrestin complexes and offer temporal control of complex assembly and dynamics

J. Janetzko, R. Kise, B. Barsi-Rhyne, D. H. Siepe, F. M. Heydenreich, M. Masureel, K. Kawakami, K. C. Garcia, M. Von Zastrow, A. Inoue, B. K. Kobilka

This study identifies membrane phosphoinositides as regulators of GPCR-arrestin complex dynamics, showing they stabilize complexes and enable temporal control of receptor signaling and recycling.

bioRxiv · Oct 9, 2021Biochemistry