M. C. Bassik

Mapping ovarian cancer spatial organization uncovers immune evasion drivers at the genetic, cellular, and tissue level

This study maps ovarian cancer spatial organization and identifies immune evasion drivers through single-cell spatial transcriptomics and CRISPR screens.

Monocytes use protrusive forces to generate migration paths in viscoelastic collagen-based extracellular matrices

This study shows that monocytes migrate through viscoelastic collagen matrices by generating protrusive forces via actin polymerization, with increased matrix stiffness and viscoelasticity enhancing their 3D migration.

A lysosomal lipid transport pathway that enables cell survival under cholinelimitation

This study identifies SPNS1 as a critical lysosomal transporter for choline recycling under limitation, revealing a novel pathway for phospholipid salvage and cell survival.

Parallel CRISPR-Cas9 screens reveal mechanisms of PLIN2 and lipid droplet regulation

The paper uses CRISPR-Cas9 screens to identify genes regulating PLIN2 and lipid droplet abundance, revealing new mechanisms and creating a resource for lipid metabolism research.

A megaprotein-based molecular bridge critical for lipid trafficking and cold resilience

This paper identifies LPD-3 as a conserved megaprotein critical for lipid trafficking between ER and PM, essential for cold resilience in C. elegans and other species.

Cell surface receptors TREM2, CD14 and integrin αMβ2 drive sinking engulfment in phosphatidylserine-mediated phagocytosis

This study identifies TREM2, CD14, and integrin αMβ2 as essential cell surface receptors for PS-mediated phagocytosis, revealing distinct mechanisms for PS and antibody-coated target uptake.

Collective invasion of the basement membrane in breast cancer driven by forces from cell volume expansion and local contractility

This study shows breast cancer cells invade the basement membrane using both proteases and mechanical forces from cell volume expansion and local contractility.

Identification of orphan ligand-receptor relationships using a cell-based CRISPRa enrichment screening platform.

The paper develops a CRISPRa-based screening platform to identify orphan ligand-receptor interactions by screening secreted proteins against cell surface receptor libraries.

Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability in cancer

The paper identifies LRP8 as a ferroptosis resistance factor in cancer, showing that its loss causes ribosome stalling during GPX4 translation, exposing a vulnerability in cancer cells.

Death-seq identifies regulators of cell death and senolytic therapies

Death-seq identifies enhancers of cell death and senolytic therapies through a CRISPR screen, revealing synergistic combinations that clear senescent cells without thrombocytopenia.

TORC1, BORC, ARL-8 cycling, and kinesin-1 drive vesiculation of cell corpsephagolysosomes

The paper identifies a molecular pathway involving TORC1, BORC, ARL-8, and kinesin-1 that drives phagolysosome vesiculation for efficient cell corpse clearance in C. elegans and mammals.

Large-scale discovery of recombinases for integrating DNA into the human genome

This paper identifies and validates new large serine recombinases (LSRs) for efficient DNA integration into human genomes, achieving higher efficiency than existing tools.

A genome-wide genetic screen uncovers novel determinants of human pigmentation

A genome-wide CRISPR-Cas9 screen identifies novel genes regulating melanogenesis, many upregulated in dark skin melanocytes and associated with skin color variation.

Spatial epitope barcoding reveals subclonaltumor patch behaviors

The paper develops a method to track subclonal tumor patch behaviors using spatial epitope barcoding and imaging, revealing emergent properties in cancer cell states.

An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput screens

The paper develops HiLITR, a tool to study protein trafficking by linking localization to reporter gene expression, and identifies genes affecting mitochondrial and ER membrane protein trafficking.

LKB1 inactivation modulates chromatin accessibility to drive metastatic progression

This study shows that LKB1 inactivation alters chromatin accessibility, promoting metastatic progression in lung cancer through SOX17 activation.

Combined Proteomic and Genetic Interaction Mapping Reveals New RAS Effector Pathways and Susceptibilities

The paper identifies new RAS effector pathways and synthetic lethal interactions in lung adenocarcinoma using proteomic and genetic screening.

Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism

The paper identifies DHODH as a target of GSK983 and shows that combining it with pyrimidine salvage inhibitors like CPU enhances antiviral efficacy against dengue virus.

The LRRC8A:C Heteromeric Channel Is a cGAMP Transporter and the Dominant cGAMP Importer in Human Vasculature Cells

The paper identifies LRRC8A:C as a cGAMP transporter in human vasculature cells and shows it mediates cGAMP import/export and is the dominant importer in these cells.

Systematic identification of regulators of antibody-drug conjugate toxicity using CRISPR-Cas9 screens

This study uses CRISPR-Cas9 screens to identify genes regulating antibody-drug conjugate (ADC) toxicity, revealing endolysosomal trafficking regulators and mechanisms of ADC resistance.

SLC19A1 is an importer of the immunotransmitter cGAMP

The paper identifies SLC19A1 as the importer of cGAMP and other CDNs, revealing its role in activating the STING pathway and informing CDN-based cancer therapy development.