Author

D. W. Watkins

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 5 Biophysics· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 7 Organism:bacteria (other)· 6 Research Focus:cell signaling & trafficking· 4 Research Focus:protein folding & dynamics· 3 Research Focus:chromatin & epigenetic regulation· 2 Research Focus:development & morphogenesis· 1 Research Focus:drug response / drug discovery· 1 Organism:E. coli· 1

Frequent Co-Authors

I. Collinson· 6 shared W. J. Allen· 5 shared A. Chacinska· 1 shared A. J. Smith· 1 shared A. P. Richardson· 1 shared A. S. F. Oliveira· 1 shared A. W. Perriman· 1 shared B. Daum· 1 shared

Activity by Year

2018-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

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6 shared papersBiochemistry

I. Collinson

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Author profile →

7 papers

Recent·Most cited

Energy landscape steering mediates dynamic coupling in ATP-driven protein translocation by the bacterial Sec machinery

J. A. Crossley, W. J. Allen, D. W. Watkins, T. Sabir, S. E. Radford, R. Tuma, I. Collinson, T. Fessl

This study uses single-molecule fluorescence to show that SecYEG dynamically couples with SecA through energy landscape steering, enhancing protein translocation efficiency.

bioRxiv · Jun 6, 2023 · PreprintBiophysics

Rate-limiting transport of positive charges through the Sec-machinery is integral to the mechanism of protein transport

W. J. Allen, R. A. Corey, D. W. Watkins, A. S. F. Oliveira, K. Hards, G. M. Cook, I. Collinson

The paper investigates protein transport through the Sec-machinery, finding that arginine diffusion limits transport and PMF contributes via a proton ratchet mechanism.

bioRxiv · Feb 2, 2022Biochemistry

A bacterial secretosome for regulated envelope biogenesis and quality control

D. W. Watkins, I. Collinson

This paper explores the biochemical mechanisms of the bacterial secretosome, focusing on its role in envelope biogenesis and quality control through interactions involving the Sec translocon and associated factors.

bioRxiv · Jan 12, 2022Biochemistry

Inter-membrane association of the Sec and BAM translocons for bacterial outer-membrane biogenesis

S. Alvira-de-Celis, D. W. Watkins, L. Troman, W. J. Allen, J. Lorriman, G. Degliesposti, E. J. Cohen, M. J. Beeby, B. Daum, V. A. M. Gold, J. M. Skehel, I. Collinson

The paper investigates the inter-membrane association between Sec and BAM translocons in bacterial outer-membrane biogenesis, showing their interaction ensures efficient OMP maturation.

bioRxiv · Jul 2, 2020Biochemistry

Controlling protein nanocage assembly with hydrostatic pressure

K. K. Le Vay, B. M. Carter, D. W. Watkins, T.-Y. D. Tang, V. P. Ting, H. Cölfen, R. P. Rambo, A. J. Smith, J. L. R. Anderson, A. W. Perriman

This study shows that hydrostatic pressure can reversibly dissociate E. coli bacterioferritin into dimers, with heme B stabilizing the cage structure.

bioRxiv · Jun 16, 2020 · PreprintBiophysics

Refined measurement of SecA-driven protein transport reveals indirect coupling to ATP turnover

W. J. Allen, D. W. Watkins, M. S. Dillingham, I. Collinson

This study uses a split luciferase assay to measure SecA-driven protein transport in bacteria, revealing stepwise transport coupled to ATP hydrolysis without a distinct initiation event.

bioRxiv · May 8, 2020 · PreprintBiochemistry

A High-Resolution Luminescent Assay For Rapid And Continuous Monitoring Of Protein Translocation Across Biological Membranes

G. C. Pereira, W. J. Allen, D. W. Watkins, L. Buddrus, D. Noone, X. Liu, A. P. Richardson, A. Chacinska, I. Collinson

This paper introduces a high-resolution luminescent assay for rapid and continuous monitoring of protein translocation, demonstrating its application in bacterial and mitochondrial systems.

bioRxiv · Nov 1, 2018Biochemistry