A random mutagenesis screen enriched for missense mutations in bacterial effector proteins.
M. L. Urbanus, T. M. Zheng, A. N. Khusnutdinova, D. Banh, H. O. Mount, A. Gupta, P. J. Stogios, A. Savchenko, R. R. Isberg, A. F. Yakunin, A. W. Ensminger
The paper presents a yeast-based method to identify informative missense mutations in bacterial effector proteins and validates it using three effectors from Legionella pneumophila.
bioRxiv · Mar 14, 2024Microbiology