Author

A. F. Yakunin

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 3 Biochemistry· 2 Bioengineering· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 7 Organism:E. coli· 3 Research Focus:protein folding & dynamics· 3 Research Focus:cell type annotation· 2 Research Focus:drug response / drug discovery· 2 Assay:metabolomics· 2 Assay:proteomics· 2 Research Focus:benchmarking· 1

Frequent Co-Authors

P. Diep· 4 shared R. Mahadevan· 4 shared A. Savchenko· 3 shared P. J. Stogios· 3 shared A. N. Khusnutdinova· 2 shared E. Evdokimova· 2 shared A. D. Hanson· 1 shared A. Gupta· 1 shared

Activity by Year

2021-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 19 mutual co-author links

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4 shared papersBiochemistry

P. Diep

Mutual co-authors in cluster

Author profile →

7 papers

Recent·Most cited

A random mutagenesis screen enriched for missense mutations in bacterial effector proteins.

M. L. Urbanus, T. M. Zheng, A. N. Khusnutdinova, D. Banh, H. O. Mount, A. Gupta, P. J. Stogios, A. Savchenko, R. R. Isberg, A. F. Yakunin, A. W. Ensminger

The paper presents a yeast-based method to identify informative missense mutations in bacterial effector proteins and validates it using three effectors from Legionella pneumophila.

bioRxiv · Mar 14, 2024Microbiology

Ni(II) binding affinity and specificity of solute binding proteins: the importance of the double His motif and variable loop revealed by structural and mutational studies

P. Diep, P. J. Stogios, E. Evdokimova, A. Savchenko, R. Mahadevan, A. F. Yakunin

This paper investigates Ni(II) binding in solute binding proteins, showing the double His motif and variable loop are critical for affinity and specificity, with implications for biocatalytic applications.

bioRxiv · Oct 3, 2022 · PreprintBiochemistry

Thermophilic carboxylesterases from hydrothermal vents of the volcanic island of Ischia active on synthetic and biobased polymers and mycotoxins

M. A. Distaso, T. N. Chernikova, R. Bargiela, C. Coscoln, P. J. Stogios, J. L. Gonzalez, S. Lemak, A. N. Khusnutdinova, F. J. Plou, E. Evdokimova, A. Savchenko, E. A. Lunev, M. M. Yakimov, O. V. Golyshina, M. Ferrer, , P. N. Golyshin

This study identifies three novel thermotolerant carboxylesterases from hydrothermal vents that hydrolyze polyesters and mycotoxins, with potential for biotechnological applications.

bioRxiv · Sep 17, 2022Microbiology

Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression

P. Diep, L. Shen, J. A. Wiesner, N. Mykytczuk, V. Papangelakis, A. F. Yakunin, R. Mahadevan

This study engineered Escherichia coli to enhance nickel bioaccumulation using overexpressed NikABCDE transporter and metallothionein, achieving 7-fold improvement but with reduced cell viability.

bioRxiv · Aug 26, 2022Bioengineering

REVOLVER: a low-cost automated protein purifier based on parallel preparative gravity column workflows

P. Diep, J. L. C. Cardenas, A. F. Yakunin, A. P. McGuigan, R. Mahadevan

REVOLVER is a low-cost automated protein purification system that enables parallel processing with minimal human intervention, achieving results comparable to manual methods.

bioRxiv · Dec 12, 2021Biochemistry

Metabolite Damage and Damage-Control in a Minimal Genome

D. Haas, A. M. Thamm, J. Sun, L. Huang, L. Sun, G. A. W. Beaudoin, K. S. Wise, C. Lerma-Ortiz, S. D. Bruner, M. Breuer, Z. Luthey-Schulten, J. Lin, M. A. Wilson, G. Brown, A. F. Yakunin, , V. de Crecy-Lagard

The paper identifies and validates genes involved in metabolite damage repair and preemption in the minimized Mycoplasma JCVI-Syn3A genome and extends its metabolic map.

bioRxiv · Dec 1, 2021Microbiology

Automation assisted anaerobic phenotyping for metabolic engineering

K. Raj, N. Venayak, P. Diep, S. A. Golla, A. F. Yakunin, R. Mahadevan

This paper develops an eco-friendly automation workflow for high-throughput anaerobic phenotyping to support metabolic engineering.

bioRxiv · May 3, 2021Bioengineering