R. R. Isberg

Loss of essential outer membrane functions causes drug hypersensitization in Acinetobacter baumannii overexpressing multidrug efflux pumps

The paper identifies essential genes in Acinetobacter baumannii that support efflux pump overproducer fitness during ciprofloxacin treatment, revealing roles in outer membrane integrity and pH homeostasis.

Identification of Determinants that Allow Maintenance of High-Level Fluoroquinolone Resistance in Acinetobacter baumannii

The paper identifies proteins essential for maintaining fitness in Acinetobacter baumannii strains overexpressing RND efflux pumps, highlighting the role of outer membrane integrity and compensatory mechanisms.

Sde Proteins Coordinate Ubiquitin Utilization and Phosphoribosylation to Promote Establishment and Maintenance of the Legionella Replication Vacuole

This study shows Sde proteins in Legionella pneumophila use deubiquitination and phosphoribosylation to manipulate host ER structure and evade autophagy.

The Sde phosphoribosyl-linked ubiquitin transferases exploit reticulons to protect the integrity of the Legionella-containing vacuole

This study shows that Legionella pneumophila uses Sde proteins to modify Rtn4, forming ER aggregates that protect the Legionella-containing vacuole from host disruption.

The Sde Phosphoribosyl-Linked Ubiquitin Transferases protect the Legionella pneumophila vacuole from degradation by the host.

This study identifies Sde proteins as temporally-regulated vacuole guards in Legionella pneumophila that prevent early vacuole disruption by blocking host endosomal trafficking.

Identification of genes required for long term survival of L. pneumophila in water.

This study identifies genes essential for Legionella pneumophila survival in water using Tn-seq and CRISPRi, showing that electron transport chain genes and cell wall stability factors are critical for long-term viability.

5' Untranslated mRNA Regions Allow Bypass of Host Cell Translation Inhibition by Legionella pneumophila

This study identifies host proteins selectively synthesized during Legionella pneumophila infection and shows that 5' untranslated regions (5UTRs) enable translation of low-abundance transcripts despite host translation inhibition.

Yersinia pseudotuberculosis YopE prevents uptake by M cells and instigates M cell extrusion in human ileal enteroid-derived monolayers.

This study uses human ileal enteroid-derived monolayers to show that Yersinia pseudotuberculosis YopE prevents uptake by M cells and causes their extrusion, facilitating bacterial transcytosis.

Members of the Legionella pneumophila Sde Family Target Tyrosine Residues for Phosphoribosyl-Linked Ubiquitination

This study shows that Legionella pneumophila Sde proteins can ubiquitinate tyrosine residues, not just serine, challenging previous assumptions about their substrate specificity.

Immunosuppression broadens evolutionary pathways to treatment failure during Acinetobacter baumannii pneumonia

This study shows that immunosuppression promotes antibiotic resistance evolution in Acinetobacter baumannii pneumonia by enabling persistence mutations that cause treatment failure without clinical resistance.

Heightened virulence of Yersinia is associated with decreased function of the YopJ protein

The study shows that reduced YopJ function in Yersinia species correlates with increased virulence in mice, suggesting that decreased cytotoxicity enhances pathogenesis.

SdhA blocks disruption of the Legionella-containing vacuole by hijacking the OCRL phosphatase

The paper shows SdhA blocks OCRL phosphatase to maintain Legionella vacuole integrity, preventing bacterial degradation.

Essential gene analysis in Acinetobacter baumannii by high-density transposon mutagenesis and CRISPR interference

This study identifies essential genes in Acinetobacter baumannii using transposon mutagenesis and a CRISPRi system, revealing new factors and conditionally essential processes.

Topologically correct synthetic reconstruction of pathogen social behavior found in deep tissue sites

The paper reconstructs microbial social behavior in deep tissue sites using microfluidics-driven microdroplets and NO generation, mimicking spatial regulation observed in tissues.

Antibiotic hypersensitivity signatures identify targets for attack in the Acinetobacter baumannii cell envelope

This study identifies antibiotic hypersensitivity signatures in A. baumannii to discover new targets for attacking the pathogen's cell envelope.

Components of the endocytic and recycling trafficking pathways interfere with the integrity of the Legionella-containing vacuole

The paper identifies host Rab GTPases and their effectors that disrupt vacuole integrity during Legionella pneumophila infection, showing their role in destabilizing the Legionella-containing vacuole.

Iron-sulfur cluster repair contributes to Y. pseudotuberculosis survival within deep tissues

This study shows that Y. pseudotuberculosis uses Fe-S cluster repair via YtfE to survive in the spleen under nitrosative stress, even when NO detoxification is absent.

Legionella pneumophila translocated translation inhibitors are required for bacterial-induced host cell cycle arrest

This study shows that Legionella pneumophila uses translation inhibitors to block host cell cycle progression and prevent entry into S phase, which is toxic for bacterial replication.