P. J. Stogios

A random mutagenesis screen enriched for missense mutations in bacterial effector proteins.

The paper presents a yeast-based method to identify informative missense mutations in bacterial effector proteins and validates it using three effectors from Legionella pneumophila.

Functional diversification despite structural congruence in the HipBST toxin-antitoxin system of Legionella pneumophila

This study characterizes a conserved TA system in Legionella pneumophila, showing functional divergence from its E. coli counterpart despite structural similarity.

Ni(II) binding affinity and specificity of solute binding proteins: the importance of the double His motif and variable loop revealed by structural and mutational studies

This paper investigates Ni(II) binding in solute binding proteins, showing the double His motif and variable loop are critical for affinity and specificity, with implications for biocatalytic applications.

Thermophilic carboxylesterases from hydrothermal vents of the volcanic island of Ischia active on synthetic and biobased polymers and mycotoxins

This study identifies three novel thermotolerant carboxylesterases from hydrothermal vents that hydrolyze polyesters and mycotoxins, with potential for biotechnological applications.

Molecular mechanism of plasmid-borne resistance to sulfonamides

This study identifies the molecular mechanism of intrinsic sulfonamide resistance in Sul enzymes through structural and functional analyses, revealing a modified active site that confers high resistance without fitness cost.

Recombinant production of growth factors for application in cell culture

This paper presents a method for producing functional growth factors in E. coli to reduce cell culture medium costs for applications like cultured meat and tissue engineering.

Structural and molecular rationale for the diversification of resistance mediated by the Antibiotic_NAT family

The paper analyzes Antibiotic_NAT family enzymes to understand resistance diversification, finding environmental variants confer broad-spectrum resistance and likely ancestral relationships to clinical genes.