Phage-encoded single-guide RNAs subvert CRISPR-Cas9 immunity
Maximilian Feussner, Angela Migur, Jinquan Li, Stephan Riesenberg, Nils Birkholz, David Mayo-Muñoz, Holly Wakelin, Chris M. Brown, Zasha Weinberg A, Rafael Pinilla‐Redondo, Peter C. Fineran, Chase L. Beisel
This paper identifies viral single-guide RNAs (vsgRNAs) that subvert bacterial CRISPR-Cas9 immunity by sequestering Cas9 and redirecting its activity, and shows they can enable genome editing in mammalian cells.
bioRxiv · Jun 2, 2026 · PreprintMicrobiology