J. E. Rothman

A proteome-wide quantitative platform for nanoscale spatially resolved extraction of membrane proteins into native nanodiscs

This paper introduces a platform for high-throughput extraction of membrane proteins into native nanodiscs while preserving their local membrane context and provides a database for optimized extraction conditions.

Unraveling cellular complexity with unlimited multiplexed super-resolution imaging

This paper introduces FLASH-PAINT, a method for rapid multiplexed super-resolution imaging of many molecular targets in mammalian cells, demonstrating its application in four cell biological studies.

Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer

This paper develops a platform for analyzing integral membrane proteins and lipids in intact membranes using native mass spectrometry, showing how lipid binding and membrane composition affect protein function.

Molecular Determinants of Complexin Clamping in Reconstituted Single-Vesicle Fusion

The paper identifies molecular mechanisms by which Complexin clamps SNARE assembly in single-vesicle fusion, showing its role in delaying fusion and enhancing clamping through specific structural interactions.

3D Adaptive Optical Nanoscopy for Thick Specimen Imaging at sub-50 nm Resolution

This paper develops a 3D adaptive optical nanoscopy technique that achieves sub-50 nm resolution in thick specimens by addressing optical aberrations.

Vesicle capture by discrete self-assembled clusters of membrane-bound Munc13

This study shows that Munc13 forms self-assembled clusters which are necessary for vesicle capture, with cluster size and the C2C domain affecting efficiency.

TANGO1 membrane helices create a lipid diffusion barrier at curved membranes

The paper shows TANGO1 membrane helices form a lipid diffusion barrier at curved membranes to prevent mixing during secretory cargo transport.

Nanoscale subcellular architecture revealed by multicolor 3D salvaged fluorescence imaging

This paper introduces a super-resolution microscope that images whole mammalian cells at ~20 nm 3D resolution, revealing complex cellular structures previously only visible via electron microscopy.