J. Bewersdorf

VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1

This study identifies VPS13B's localization at the cis-trans Golgi interface and shows its functional interaction with FAM177A1 in lipid transport and Golgi dynamics.

Unraveling cellular complexity with unlimited multiplexed super-resolution imaging

This paper introduces FLASH-PAINT, a method for rapid multiplexed super-resolution imaging of many molecular targets in mammalian cells, demonstrating its application in four cell biological studies.

The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ

The paper shows Rtn4 oligomers increase ER membrane curvature by localizing to elliptical tubules and enhancing local density.

Extracting nanoscale membrane morphology fromsingle-molecule localizations

The paper introduces NanoWrap, a method for reconstructing subcellular membrane surfaces from single-molecule localization microscopy data, validated on simulated and experimental data.

Unclearing Microscopy

This paper introduces a new microscopy technique called Unclearing Microscopy that allows visualization of subcellular structures with the unaided eye by expanding cells and opaquing them with light-scattering molecules.

An Integrated Platform for High-Throughput Nanoscopy

This paper presents an integrated platform for high-throughput single-molecule localization microscopy (SMLM) that enables imaging of 10,000 cells per day.

Cryo-ET of a human GBP coatomer governing cell-autonomous innate immunity to infection

The paper solves the native structure of a human GBP coatomer that coats cytosolic bacteria and functions as an antimicrobial signaling platform.

Extremely bright, near-IR emitting spontaneously blinking fluorophores enable ratiometric multicolor nanoscopy in live cells

The paper introduces a new near-IR fluorophore that enables two-color live-cell SMLM without chemical additives or multiple lasers.

Multi-omics profiling of U2AF1 mutants dissects pathogenic mechanisms affecting RNA granules in myeloid malignancies

This study uses multi-omics profiling to analyze U2AF1 mutations in myeloid malignancies, revealing altered RNA binding, splicing, and stress granule formation.

3D Adaptive Optical Nanoscopy for Thick Specimen Imaging at sub-50 nm Resolution

This paper develops a 3D adaptive optical nanoscopy technique that achieves sub-50 nm resolution in thick specimens by addressing optical aberrations.

DMA-tudor interaction modules control the specificity of in vivo condensates

The paper shows that DMA-tudor interactions drive the formation of specific membraneless organelles by controlling condensate assembly and composition.

Light microscopy of proteins in their ultrastructural context

This paper introduces a method to expand cellular structures while preserving proteins, enabling efficient labeling and visualization of protein distributions at the nanoscale using standard light microscopy.

Circumventing the optical diffraction limit with customized speckles

This study demonstrates that customized speckle patterns can achieve three times higher spatial resolution than the diffraction limit in parallelized nonlinear pattern-illumination microscopy.

Sample preparation and imaging conditions affect mEos3.2 photophysics in fission yeast cells

This study investigates how formaldehyde fixation and imaging conditions affect the photophysics of mEos3.2 in fission yeast cells, providing a guide for quantitative imaging and counting of mEos3.2-tagged molecules.

Fluorogenic probe for fast 3D whole-cell DNA-PAINT

This paper introduces a fluorogenic DNA-PAINT probe that reduces background and increases imaging speed for 3D whole-cell DNA-PAINT.

Accurate 4Pi single-molecule localization using an experimental PSF model

The paper develops a method to improve 4Pi single-molecule localization microscopy by fitting an experimental PSF model, achieving the theoretical resolution limit.

3D super-resolution deep-tissue imaging in living mice

This paper develops a STED system for 3D super-resolution imaging in living mice, achieving depths of 76 micrometers in brain tissue.

Nanoscale subcellular architecture revealed by multicolor 3D salvaged fluorescence imaging

This paper introduces a super-resolution microscope that images whole mammalian cells at ~20 nm 3D resolution, revealing complex cellular structures previously only visible via electron microscopy.

A stable, high refractive index, switching buffer for super-resolution imaging

The paper develops a stable, high refractive index buffer for dSTORM super-resolution imaging, improving stability, switching speed, contrast, and refractive index matching.

EFCAB9 is a pH-Dependent Ca2+ Sensor that Regulates CatSper Channel Activity and Sperm Motility

This study identifies EFCAB9 as a pH-dependent Ca2+ sensor that modulates CatSper channel activity and sperm motility through its interaction with CATSPERζ.

Simultaneously measuring image features and resolution in live-cell STED images

The paper introduces NEP fitting to measure resolution and cellular features in live-cell STED images without beads or pre-calibration, validated on microtubules and applied to ER tubules.

Analysis Of Interphase Node Proteins In Fission Yeast By Quantitative And Super Resolution Fluorescence Microscopy

The paper uses microscopy techniques to study interphase nodes in fission yeast, finding they have defined sizes and protein compositions, with type 1 nodes disassembling during mitosis and type 2 nodes remaining intact.