A putative rhamnogalacturonan-II CMP-β-Kdo transferase identified using CRISPR/Cas9 gene edited callus to circumvent embryo lethality.
Y. Zhang, D. Sharma, Y. Liang…·J. C. Mortimer
The paper identifies a putative rhamnogalacturonan-II CMP-β-Kdo transferase by using CRISPR/Cas9 to generate a callus mutant lacking Kdo, which disrupts RG-II crosslinking and growth.