K. Holden

Rapid generation of precision preclinical cancer models using regulatable in vivo base editing

This study develops a murine system for temporal and regulatable in vivo cytosine base editing to rapidly generate preclinical cancer models with specific SNVs.

Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrases

PASTE enables efficient, programmable gene integration without DNA breaks, applicable for research, cell engineering, and gene therapy.

Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells

The paper identifies chemically modified crRNAs that enhance CRISPR-Cas13 knockdown efficiency and half-life in human cells.

Identification of DAXX As A Restriction Factor Of SARS-CoV-2 Through A CRISPR/Cas9 Screen

The paper identifies DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen, showing it limits viral replication independently of SUMOylation.

BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2

This study identifies BRD2 as a regulator of ACE2 transcription and shows that BRD2 inhibitors block SARS-CoV-2 infection in human cells and animal models.

Phenotypic and environmental predictors of reproductive success in painted turtles

This study examines predictors of reproductive success in painted turtles, finding that female traits like body size and corticosterone levels predict reproductive success, while male traits and biased sex ratios have weaker effects.

Functional genomic screens identify human host factors for SARS-CoV-2 and common cold coronaviruses

The paper identifies host factors and pathways common to SARS-CoV-2 and common cold coronaviruses using CRISPR screens, revealing shared and unique factors that support viral infection.