Manipulating the murine Lgr5 locus using a rapid, efficient and flexible CRISPR/Cas9 pipeline
Jan Reichmuth, Johannes vom Berg, Michael David Brügger, George Hausmann, Tomáš Valenta, Konrad Basler
The paper introduces a CRISPR/Cas9-based mouse model for lineage tracing and gene manipulation in Lgr5+ intestinal stem cells without affecting endogenous Lgr5 function.
bioRxiv · Dec 31, 2020 · PreprintDevelopmental Biology