iAPEX: Improved APEX-based proximity labeling for subcellular proteomics using an enzymatic reaction cascade
Tommy J Sroka, Lea K. Sanwald, Avishek Prasai, Josefine Hoeren, Karina von der Malsburg, Valerie Chaumet, Per Haberkant, Kerstin Feistel, David U. Mick
The paper introduces iAPEX, an improved APEX-based proximity labeling method for live-cell proteomics, and applies it to study primary cilia in Xenopus laevis, identifying new ciliary proteins and validating existing ones.
bioRxiv · Jan 10, 2025 · PreprintBiochemistry