John B. Wallingford

This study shows ODAD1 is essential for ODA-DC assembly in 9+0 and 9+2 cilia, causing left-right patterning and ciliary motility defects in ciliopathy.

The paper predicts the structure of the CPLANE complex and identifies novel interactors of Jbts17, revealing its role in ciliogenesis through in silico and experimental validation.

CiliaIO uses machine learning to quantify cilia beating dynamics in zebrafish spinal cord, revealing regional waveform differences and subtle defects in a bbs2 mutant.

The paper reconstructs the protein interactome of the Last Eukaryotic Common Ancestor and identifies new human gene-disease associations for bone density and congenital birth defects.

The paper identifies Jhc1, a ciliary protein essential for cilia structure and length in Xenopus, and shows its evolutionary origin and functional adaptation.

The paper identifies two proteins, Ccdc78 and Ccdc33, at the distal tip of vertebrate motile cilia, showing they are essential for cilia organization, length regulation, and function.

The paper investigates the role of Lrrc56 in vertebrate multiciliated cells, showing it regulates outer dynein arm deployment through interaction with Odad3, linking LRRC56 mutations to primary ciliary dyskinesia.

This study identifies RSG1/CPLANE2 as a causative gene for human ciliopathy and shows its role in ciliary transition zone assembly.

The paper shows that a nematic liquid crystal state forms during gastrulation in fish, frogs, and fruit flies through a common mechanism, with experimental and theoretical support.

This study shows that the LIM domain of Prickle2 binds stressed actin filaments, but full-length Prickle2 does not, and identifies mutations that disrupt this interaction.

The paper uses whole-cell proteomics to identify novel regulators of ciliogenesis in Xenopus multiciliated cells, revealing 832 upregulated proteins involved in ciliary structures and function.

The paper identifies de novo mutations contributing to meningomyelocele risk by analyzing trios and finding damaging mutations in 22.3% of cases.

The paper investigates how polarized cellular forces during 4-cell rosette resolution drive tissue-scale convergent extension by enabling force propagation and proper cell packing.

The paper investigates the role of Kif6 in ependymal cilia motility and polarity, finding that its loss causes decreased cilia movement and hydrocephalus in mice.

The paper presents high-quality genomes and chromatin structure analyses of frogs, revealing conserved ancestral chromosomes and stable genome evolution.

The paper investigates the role of PCP and Septins in organizing the actin cytoskeleton during Xenopus convergent extension.

The paper introduces two non-invasive techniques for subcellular biomechanical analysis in vivo, revealing PCP-dependent mechanical gradients during convergent extension.

This study identifies Hedgehog signaling as essential for cyclical uterine remodeling in mice, showing that its disruption prevents remodeling and causes smooth muscle hypertrophy.

This study investigates the role of Plekhg5 in regulating medioapical actomyosin dynamics during Xenopus gastrulation, showing its critical role in apical constriction.

The paper quantifies the relative rates of axonemal deployment for cilia beating machineries during the final stages of differentiation in Xenopus multiciliated cells.

The paper uses in vivo imaging and regression analysis to show that Shroom3 has non-cell autonomous effects on apical constriction and N-cadherin localization during neural tube closure.

This study shows that convergent extension involves both cell crawling and junction contraction, which work together more effectively when integrated through mechano-reciprocity, and introduces a computational model to explore their biomechanical interactions.

The paper uses tissue-level time-lapse microscopy to study cell behavior and protein localization during Xenopus neural tube closure, revealing region-specific functions of Shroom3 and N-cadherin.

The paper investigates how Arvcf catenin links subcellular actomyosin dynamics to tissue-scale force production during vertebrate convergent extension.

hu.MAP 2.0 integrates over 15,000 proteomic experiments to identify nearly 7,000 human protein complexes, improving accuracy and uncovering new biological insights.

The paper analyzes the spatiotemporal gene expression patterns in the mouse oviduct across the estrous cycle and anteroposterior axis, finding stable transcriptional dynamics across the cycle.

The paper investigates the role of Lrp2 in neural tube closure using mouse and Xenopus models, identifying its interaction with Shroom3 and Gipc1 and its role in apical constriction and planar cell polarity.

The paper provides a time-resolved bulk transcriptome of developing Keller explants from Xenopus laevis dorsal marginal zone.

This study identifies TUBB8 as the molecular target of benzimidazoles, revealing their mechanism of vascular disruption and potential clinical applications.

The paper introduces DIF-FRAC, a label-free method to identify RNA-associated proteins in vertebrate tissues, revealing novel RAPs and RNA-enriched organelles.