K. Minamihata

Engineered Active Zymogen of Microbial Transglutaminase

This paper designs an engineered active zymogen of microbial transglutaminase (EzMTG) that exhibits cross-linking activity without requiring proteolysis, and identifies mutations that balance binding affinity and catalytic activity.

Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25 by silkworm, Bombyx mori, and its biochemical analysis.

This study expresses and purifies recombinant Pvs25 from silkworms, demonstrating its potential as a malaria transmission-blocking vaccine candidate with high immunogenicity.

Recombinant production of active microbial transglutaminase in E. coli using self-cleavable zymogen with mutated propeptide

This paper describes the production of active microbial transglutaminase in E. coli using a modified zymogen with mutations in the propeptide to achieve pure, active enzyme.