K. Stejskal

Metabolic enzymes moonlight as selective autophagy receptors to protect plants against viral-induced cellular damage

This study shows that metabolic enzymes act as autophagy receptors in plants to degrade defense executors during viral infection, revealing autophagy as a tolerance pathway rather than an antiviral defense.

Mast cell-derived BH4 is a critical mediator of postoperative pain

This study identifies mast cell-derived BH4 as a key mediator of postoperative pain, showing that substance P triggers BH4-dependent serotonin release in mast cells, and blocking substance P reduces pain.

Wide Window Acquisition and AI-based data analysis to reach deep proteome coverage for a wide sample range, including single cell proteomic inputs

The paper introduces a workflow combining micro pillar array columns, wide window acquisition, and AI-based search engine to enhance proteome coverage and throughput, particularly for single-cell samples.

Deep proteome profiling with reduced carry over using superficially porous microfabricated nanoLC columns

This paper evaluates prototype nanoLC columns for improved proteome profiling, finding increased protein group and peptide identification with reduced carry over and methionine oxidation.

Quantitative accuracy and precision in multiplexed single-cell proteomics.

This study evaluates the impact of carrier spikes on quantitative accuracy in multiplexed single-cell proteomics and proposes optimized experimental designs.

Comparative proteome signatures of trace samples by multiplexed Data-Independent Acquisition

The paper develops a method to quantitatively compare proteome signatures from ultra-low input samples using DIA-TMT multiplexing and a novel analysis pipeline.

Ultra-sensitive nanoLC-MS of sub nanogram protein samples using second generation micro pillar array LC technology with Orbitrap Exploris 480 and FAIMS PRO

This paper benchmarks ultra-sensitive proteomics methods using micro pillar array LC and FAIMS PRO, achieving detection of 1486 protein groups from 250 pg of HeLa tryptic digest.