Author

Karen L Kanke

Nationwide Children's Hospital

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Genetics· 1 Molecular Biology· 1

Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 2 Research Focus:drug response / drug discovery· 2 Approach:experimental assay· 2 Research Focus:gene regulatory network inference· 2 Organism:human· 2 Research Focus:variant calling· 2 Research Focus:perturbation response prediction· 1

Frequent Co-Authors

Estelle Cormet‐BoyakaiD· 2 shared Jacob T. StackiD· 2 shared Rachael E. RayneriD· 2 shared Reza NouriiD· 2 shared Sriram VaidyanathaniD· 2 shared Tatyana A. VetteriD· 2 shared Aparna VenugopalaniD· 1 shared Carlos Jose SuareziD· 1 shared

Activity by Year

2024-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 42 mutual co-author links

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2 shared papersGenetics

Estelle Cormet‐BoyakaORCID

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2 papers

Recent·Most cited

DNA-PKcs Inhibition Improves Sequential Gene Insertion of the Full-Length CFTR cDNA in Airway Stem Cells

Jacob T. Stack, Rachael E. Rayner, Reza Nouri, Carlos Jose Suarez, Sun Hee Kim, Karen L Kanke, Tatyana A. Vetter, Estelle Cormet‐Boyaka, Sriram Vaidyanathan

This study shows that inhibiting DNA repair pathways with AZD7648 improves gene insertion efficiency in airway stem cells for CFTR correction, but may introduce toxicity.

Molecular Therapy — Nucleic Acids · Sep 17, 2024Peer-reviewedGenetics

Single-Stranded DNA with Internal Base Modifications Mediates Highly Efficient Gene Insertion in Primary Cells

Karen L Kanke, Rachael E. Rayner, Eli Abel, Aparna Venugopalan, Ma Suu, Jacob T. Stack, Reza Nouri, Gongbo Guo, Tatyana A. Vetter, Estelle Cormet‐Boyaka, Mark E. Hester, Sriram Vaidyanathan

The paper demonstrates that chemically modified ssDNA (eDNA) significantly improves gene insertion efficiency in primary cells compared to unmodified ssDNA, achieving therapeutic levels in clinically relevant loci.

bioRxiv · Feb 1, 2024 · PreprintMolecular Biology