Higher resolution pooled genome-wide CRISPR knockout screening in Drosophila cells using integration and anti-CRISPR (IntAC)
Raghuvir Viswanatha, Samuel W. Entwisle, Yanhui Hu, Ah‐Ram Kim, Kelly Reap, Matthew Butnaru, Mujeeb Qadiri, Stephanie Elizabeth Mohr, Norbert Perrimon
The paper introduces IntAC, a CRISPR screening method for Drosophila cells that improves accuracy by suppressing early Cas9 activity, leading to a more comprehensive list of fitness genes.
Nature Communications · Jul 15, 2025Peer-reviewedGenetics