Raghuvir Viswanatha

ABC transporters support Drosophila intestinal stem and progenitor cells during cytotoxic challenge

The paper shows that ABC transporters in Drosophila intestinal stem/progenitor cells reduce compound accumulation and enhance survival during cytotoxic stress.

Intersecting experimental evolution and CRISPR screens to identify novel toxin resistance loci

The paper identifies genes involved in octanoic acid resistance by combining experimental evolution and CRISPR screens in Drosophila species.

A pro-apoptotic selection strategy enables CRISPR screening in mosquitoes and identifies Lachesin as a chikungunya virus entry factor

The paper uses a pro-apoptotic CRISPR screening strategy in mosquito cells to identify Lachesin as a critical CHIKV entry factor.

Lachesin is a mosquito receptor for multiple arthritogenic alphaviruses

This study identifies Lachesin as a mosquito receptor for multiple arthritogenic alphaviruses using CRISPR screening in Aedes albopictus cells.

Genome-wide CRISPR knockout cell screening platform for the disease vector tick species Ixodes scapularis

The paper develops a genome-wide CRISPR-Cas9 knockout screening platform for Ixodes scapularis ticks and identifies genes associated with cellular fitness and drug resistance.

A family of lethal exotoxins defined by cell entry via the Attractin receptor

The paper identifies Attractin as the receptor for Nigritoxin, a Vibrio toxin, and shows that related toxins use this receptor but have distinct effector domains.

A Genome-wide CRISPR Screen Reveals ZDHHC8-Dependent Gαq Palmitoylation as a Key Regulator of GPCR Signaling

The paper identifies ZDHHC8-dependent Gαq palmitoylation as a critical regulator of GPCR signaling through a genome-wide CRISPR screen in Drosophila.

Higher resolution pooled genome-wide CRISPR knockout screening in Drosophila cells using integration and anti-CRISPR (IntAC)

The paper introduces IntAC, a CRISPR screening method for Drosophila cells that improves accuracy by suppressing early Cas9 activity, leading to a more comprehensive list of fitness genes.

A genome-wide CRISPR screen in Anopheles mosquito cells identifies essential genes and required components of clodronate liposome function

The paper uses a genome-wide CRISPR screen in Anopheles mosquito cells to identify essential genes and factors involved in clodronate liposome function.

Modular vector assembly enables rapid assessment of emerging CRISPR technologies

The paper introduces a modular CRISPR vector assembly system called Fragmid, enabling rapid creation and testing of CRISPR technologies through combinatorial design and pooled screening.

Pooled genome-wide CRISPR activation screening for rapamycin resistance genes in Drosophila cells

The paper develops a pooled CRISPR activation screening platform in Drosophila cells to identify rapamycin resistance genes, finding three novel genes involved in RTK-Akt-mTOR pathway activation.

A genome-wide CRISPR screen identifies the glycosylation enzyme DPM1 as a modifier of DPAGT1 deficiency and ER stress

The paper identifies DPM1 as a therapeutic target for DPAGT1-CDG by showing its inhibition rescues ER stress and glycoprotein levels in Drosophila models.

Local assembly of long reads enables phylogenomics of transposable elements in a polyploid cell line

The paper develops a method to analyze transposable elements in polyploid genomes using long-read sequencing, revealing somatic transposition events and TE family proliferation.

Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

The paper develops bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquito cell lines to study gene function and disease control.

CRISPR-Cas13 mediated Knock Down in Drosophila cultured cells.

This study develops and optimizes CRISPR-Cas13 tools for RNA knockdown in Drosophila cells, improving efficiency and reducing toxicity.

SNP-CRISPR: a web tool for SNP-specific genome editing

SNP-CRISPR is a web tool that designs CRISPR sgRNAs for SNP-containing alleles, accounting for genetic variations in non-reference genomes and across genetic backgrounds.

Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells

The paper develops a site-specific integration strategy for CRISPR screening in Drosophila cells, identifying essential genes and context-specific fitness regulators.