L. Limozin

Antigen density and applied force control enrichment of nanobody-expressing yeast cells in microfluidics

This paper develops a microfluidic assay to select yeast-displayed nanobodies based on antigen density and applied force, enabling enrichment of binders with both high affinity and force resistance.

Probing mechanical interaction of immune receptors and cytoskeleton by membrane nanotube extraction

This study uses mechanical extraction and modeling to investigate the mechanical link between immune receptors and the cytoskeleton, revealing that TCR-complex and open LFA1 form such links.

Combining DNA scaffolds and acoustic force spectroscopy to characterize individual protein bonds

The paper combines DNA scaffolds and acoustic force spectroscopy to measure single-molecule protein bond rupture under force, showing agreement with standard techniques.

Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-rates

The paper shows mechanical forces reduce T cell antigen discrimination by accelerating off-rates of higher-affinity antigens.

Controlling T cells shape, mechanics and activation by micropatterning.

This paper demonstrates a method to control T cell shape, mechanics, and activation using micropatterned substrates and shows that adhesion to aCD45 does not affect calcium fluxes.

CD8 co-receptor enhances T cell activation without any effect on initial attachment

This study investigates how CD8 co-receptor affects T cell activation by analyzing TCR-pMHC interactions using single-molecule techniques, finding that CD8 enhances spreading responses rather than initial binding.

Biphasic mechanosensitivity of TCR mediated adhesion of T lymphocytes

The paper shows T cells exhibit biphasic adhesion responses to environmental stiffness via TCR, with maximal adhesion on optimally stiff surfaces, and identifies key molecular parameters determining this behavior.