J. Pettmann

Using CombiCells, a platform enabling titration and combinatorial display of cell surface ligands, to study T cell antigen sensitivity by TCRs, CARs, and BiTEs

The paper uses CombiCells to compare T cell antigen sensitivity mediated by TCRs, CARs, and BiTEs and assess the impact of co-stimulation and co-inhibition receptors.

Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-rates

The paper shows mechanical forces reduce T cell antigen discrimination by accelerating off-rates of higher-affinity antigens.

T-cell microvilli simulations show operation near packing limit and impact on antigen recognition.

The paper develops a model of T-cell microvilli dynamics to show they operate near packing limits and have a modest impact on antigen discrimination.

Inefficient exploitation of accessory receptors reduces the sensitivity of chimeric antigen receptors.

This study shows that CARs have lower antigen sensitivity than TCRs due to inefficient use of accessory receptors, and demonstrates ways to improve this sensitivity.

Differential impact of TNFRSF co-stimulation on CD8+ T cell cytokine production by feedback control of surface receptor expression

This study investigates how different TNFRSF co-stimulation receptors affect CD8+ T cell cytokine production, revealing that feedback control of surface receptor expression explains their differential impact.

The discriminatory power of the T cell receptor

This study measures TCR discriminatory power against pMHC antigens and finds it is imperfect, with T cells responding to low-affinity self pMHC with KD ~1 mM.

T cells exhibit unexpectedly low discriminatory power and can respond to ultra-low affinity peptide-MHC ligands

The paper measures ultra-low TCR/pMHC affinities and finds T cells have low discriminatory power, requiring large affinity differences to trigger responses.

Human CD8+ T cells exhibit a shared antigen threshold for different effector responses

The paper shows that different effector responses of human CD8+ T cells share a common antigen threshold across varying affinities and co-stimulatory conditions.

Perfect adaptation of CD8+ T cell responses to constant antigen input over a wide range of affinity is overcome by costimulation

The paper shows that partial TCR downregulation in T cells leads to perfect adaptation to constant antigen stimulation, which can be overcome by costimulation.