S. J. J. Brouns

High resolution reconstruction of a Jumbo bacteriophage infecting capsulated bacteria using hyperbranched tail fibers

This study determines the high-resolution structure of a jumbo bacteriophage infecting Klebsiella pneumoniae, revealing its complex tail fibers that enable infection of multiple capsular polysaccharide types.

SCOPE: Flexible targeting and stringent CARF activation enables type III CRISPR-Cas diagnostics

This study identifies key regions in type III CRISPR-Cas systems for RNA targeting and cOA production, leading to the development of the SCOPE diagnostic tool for SARS-CoV-2 detection.

Single Cell Variability of CRISPR-Cas Interference and Adaptation

The paper studies CRISPR-Cas interference and adaptation in E. coli, finding cell-to-cell variability in clearance times due to primed adaptation, with Cascade binding as the main source of heterogeneity.

Extracting transition rates in single-particle tracking using analytical diffusion distribution analysis

This paper introduces anaDDA, a method to extract transition rates from diffusion coefficient distributions in single-particle tracking, showing improved performance in fast regimes and applications to DNA polymerase I in E. coli.

CRISPR-based DNA and RNA detection with liquid-liquid phase separation

This paper introduces a label-free CRISPR-based method for nucleic acid detection using liquid-liquid phase separation, showing that target sequence cleavage reduces turbidity.

An open microscopy framework suited for tracking dCas9 in live bacteria

The paper introduces a low-cost super-resolution microscope for tracking dCas9 in live bacteria and quantifies its binding kinetics.

Repetitive DNA reeling by the Cascade-Cas3 complex in nucleotide unwinding steps

This study investigates the DNA unwinding mechanism of the Cascade-Cas3 complex using single-molecule FRET, revealing a spring-loaded reeling process that compensates for inefficient nicking.