Interference Requirements of Type III CRISPR-Cas Systems from Thermus thermophilus
K. Karneyeva, M. Kolesnik, A. Livenskyi, V. Zgoda, V. Zubarev, A. Trofimova, D. Artamonova, Y. Ispolatov, K. Severinov
This study investigates the interference requirements of Type III-A and III-B CRISPR-Cas systems from Thermus thermophilus, finding that target RNA abundance influences crRNA-target complementarity needed for interference.
bioRxiv · Sep 3, 2023 · PreprintMicrobiology