M. W. Martynowycz

This study uses MicroED and cryo-FIB milling to determine a 1.10 Å resolution structure of d(CGCGCG)2 DNA crystals.

This paper determines the structure of the human vasopressin 1B receptor using MicroED, overcoming challenges in GPCR structural determination.

This paper introduces a method to prepare lipidic cubic phase (LCP) membrane protein crystals for MicroED by using fluorescence microscopy to identify and mill crystals without converting LCP to sponge phase.

This paper demonstrates the use of K2 and K3 direct electron detectors in electron-counting mode for MicroED data collection, enabling structure determination of macromolecules at resolutions between 1.2 and 2.8 Å.

This study uses MicroED to identify hydrogen atom positions and hydrogen bonding networks in proteins, providing detailed structural insights for drug discovery and structural biology.

The paper determines the structures of two proteins using ab initio phasing with MicroED data collected on a direct electron detector in counting mode.

This study benchmarks the optimal thickness for cryo-EM samples using MicroED, finding that structures can be reliably determined up to twice the inelastic mean free path.

The paper determines the structure of the human A2A adenosine receptor using MicroED after converting LCP to sponge phase and cryoFIB milling, achieving 2.8 Å resolution.

The paper determines the near-atomic structure of mouse VDAC using MicroED after milling microcrystals grown in a lipid matrix.

This paper introduces a method for efficiently soaking ligands into protein microcrystals on TEM grids, achieving higher ligand occupancy and resolution compared to traditional X-ray crystallography.

The paper determines the structure of a seven-residue peptide using MicroED by analyzing radiation damage-induced differences in diffraction data.

This study compares serial X-ray crystallography and MicroED for determining structures from small macromolecular crystals, highlighting their strengths and challenges.

This study evaluates two electron detectors for MicroED, showing the Falcon III reduces radiation damage and improves data collection speed and efficiency.

The paper describes a procedure for automated MicroED data collection using SerialEM, enabling high-throughput data collection of over 300 datasets overnight.