I. D. Young

Changes in an Enzyme Ensemble During Catalysis Observed by High Resolution XFEL Crystallography

The paper uses XFEL crystallography to observe conformational changes in an enzyme during catalysis, revealing ionization-linked dynamics that control catalytic activity.

Structural characterization of ligand binding and pH-specific enzymatic activity of mouse Acidic Mammalian Chitinase

This study characterizes the pH-specific enzymatic activity and ligand binding of mouse AMCase using structural and computational methods, revealing dual activity optima at pH 2 and 7.

Mapping Protein Dynamics at High-Resolution with Temperature-Jump X-ray Crystallography

This study uses temperature-jump X-ray crystallography to observe atomic-scale protein motions in lysozyme, showing how vibrations evolve into functional movements and how inhibitors alter these dynamics.

Structural basis for context-specific inhibition of translation by oxazolidinone antibiotics

This study determines cryo-EM structures of oxazolidinone antibiotics bound to stalled ribosomes, revealing how alanine at the penultimate position enables context-specific inhibition.

CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes.

The paper uses cryo-EM and AlphaFold2 to determine the structure of SARS-CoV-2 Nsp2, revealing its zinc-binding site and potential interactions with host proteins.

Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance

This study uses directed evolution to identify Cfr variants that enhance rRNA methylation, revealing structural insights into antibiotic resistance mechanisms.

Fragment binding to the Nsp3 macrodomain of SARS-CoV-2 identified through crystallographic screening and computational docking

The paper identifies new chemical fragments binding to the SARS-CoV-2 Nsp3 macrodomain through crystallographic screening and computational docking, validating their binding with biophysical techniques.

Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

This study compares serial X-ray crystallography and MicroED for determining structures from small macromolecular crystals, highlighting their strengths and challenges.