J. Hattne

Eliminating the missing cone challenge through innovative approaches

This paper demonstrates that suspended drop crystallization eliminates the missing cone challenge in MicroED for thin plate crystals.

Electron-counting in MicroED

This paper discusses the benefits of electron-counting detectors in MicroED, highlighting their ability to improve data recording speed and accuracy while reducing radiation damage concerns.

A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals

This paper introduces a method to prepare lipidic cubic phase (LCP) membrane protein crystals for MicroED by using fluorescence microscopy to identify and mill crystals without converting LCP to sponge phase.

Electron-counting MicroED data with the K2 and K3 direct electron detectors

This paper demonstrates the use of K2 and K3 direct electron detectors in electron-counting mode for MicroED data collection, enabling structure determination of macromolecules at resolutions between 1.2 and 2.8 Å.

Hydrogens and hydrogen-bond networks in macromolecular MicroED data

This study uses MicroED to identify hydrogen atom positions and hydrogen bonding networks in proteins, providing detailed structural insights for drug discovery and structural biology.

Ab initio phasing macromolecular structures using electron-counted MicroED data

The paper determines the structures of two proteins using ab initio phasing with MicroED data collected on a direct electron detector in counting mode.

Benchmarking ideal sample thickness in cryo-EM using MicroED

This study benchmarks the optimal thickness for cryo-EM samples using MicroED, finding that structures can be reliably determined up to twice the inelastic mean free path.

MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP

The paper determines the structure of the human A2A adenosine receptor using MicroED after converting LCP to sponge phase and cryoFIB milling, achieving 2.8 Å resolution.

MicroED structure of lipid-embedded mammalian mitochondrial voltage dependent anion channel

The paper determines the near-atomic structure of mouse VDAC using MicroED after milling microcrystals grown in a lipid matrix.

Experimental phasing of MicroED data using radiation damage

The paper determines the structure of a seven-residue peptide using MicroED by analyzing radiation damage-induced differences in diffraction data.

MicroED with the Falcon III direct electron detector

This study evaluates two electron detectors for MicroED, showing the Falcon III reduces radiation damage and improves data collection speed and efficiency.

Qualitative analyses of polishing and pre-coating FIB milled crystals for MicroED

This study evaluates polishing and pre-coating techniques for FIB-milled crystals in MicroED, finding that platinum pre-coating and polishing improve signal-to-noise ratio, resolution, and refinement statistics.

MicroED data collection with SerialEM

The paper describes a procedure for automated MicroED data collection using SerialEM, enabling high-throughput data collection of over 300 datasets overnight.

Collection of continuous rotation MicroED Data from Ion Beam Milled Crystals of Any Size

This paper introduces a method to prepare protein crystals for continuous rotation MicroED using ion beam milling, eliminating the need for blotting optimization.

Analysis of global and site-specific radiation damage in cryo-EM

The paper analyzes radiation damage in cryo-EM using MicroED, finding similar damage dynamics to X-ray crystallography, including metal ion perturbation and disulfide bond breaking.

MicroED Structures of HIV-1 Gag CTD-SP1 Reveal Binding Interactions with the Maturation Inhibitor Bevirimat

The paper uses MicroED to determine the structure of HIV-1 Gag CTD-SP1 with bevirimat, revealing binding interactions that inform drug discovery.